绿帝王组织培养快速繁殖技术

选取绿帝王盆栽大苗作为供试母株,对绿帝王组织培养快速繁殖技术进行了研究。结果表明:绿帝王组培外植体最佳取材时间应在冬、春季;不定芽分化增殖阶段最佳培养基为M S十6-BA 3.0m g/L十NAA 0.3 m g/L;最佳生根培养基为1/2M S十NAA 0.5 m g/L十蔗糖20 g/L十活性碳2.0 g/L,和1/2M S十IBA 2.0 m g/L十蔗糖20 g/L十活性碳2.0 g/L;苗高达4 cm,有4片以上叶子,1条~3条以上根系的健壮试管苗,放温室进行3 d~15 d的闭瓶炼苗,7 d~10 d开瓶炼苗,移栽成活率可达95%以上。

Rapid Micro-propagation Techniques for Philodendron erubescens ‘Ludiwang’

Using Philodendron erubescens 'Ludiwang' seedling-in-pot as test material,its micro-propagation techniques was obtained.The results showed that Winter and Spring are the optimum time for explant;Differentiation of adventitious bud was in MS medium culture with 6-BA3.0 mg/L NAA0.3 mg/L;The best medium for rooting was 1/2MS NAA0.5 mg/L sucrose20 g/L activated carbon2.0 g/L and 1/2MS IBA2.O mg/L sucrose20 g/L activated carbon 2.0 g/L;The plantlets,with height of 4 cm,leaves of 4 or above,roots of 3 or 4,was cultured in sealed bottle in greenhouse for 3-15 d,then trained in sealed bottle for 7-10 d,so the survival rate of transplanted plantlets was over 95%.