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Cloning and Analysis of Full-Length cDNA of PumNPR1 Gene from Pyrus ussuriensis Maxim |
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| Authors: | CHE Daidi FAN Jinping WANG Jingang XU Ping YANG Tao LIU Shenkui |
| Affiliation: | 1. College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China;College of Horticulture, Northeast Agricultural University, Harbin 150030, China 2. College of Horticulture, Northeast Agricultural University, Harbin 150030, China 3. College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China |
| Abstract: | The purpose of this study is to find a new gene resource for the researches of molecular breeding of Rosaceae plants disease-resistance. Pyrus ussuriensis Maxim is used as a starting material to clone the full-length cDNA of NPR1(nonexpressor of pathogenesis- related genes 1) which is a key regulator in SA (salicylic acid)-mediated systemic acquired resistance (SAR) by homologous cloning and RACE techniques. The length of the cDNA sequence was 1 767 bp, the ORF was 1 761 bp, it coded 586 amino acids, pI=5.58, the relative molecular weight was 65.009 ku, contained 19 kinds of amino acids, and had full BTB/POZ and ANK domains. Compared the homology of NPR1 gene in GenBank database, the homology with Pyrus pyrifolia, Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon esculentum, Oryza sativa, Helianthus annuus were 98%, 62%, 68%, 65%, 57%, 63%. The homology of functional area were 99%, 78%, 82%, 79%, 74%, 77%. This NPR1 gene was considered as homologic gene of Pyrus ussuriensis Maxim and named PumNPR1. |
| Keywords: | Pyrus ussuriensis Maxim NPR1 gene cloning RACE |
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