Rapid Sorting of Fucoxanthin-Producing Phaeodactylum tricornutum Mutants by Flow Cytometry |
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| Authors: | Yong Fan Xiao-Ting Ding Li-Juan Wang Er-Ying Jiang Phung Nghi Van Fu-Li Li |
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| Institution: | 1.Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; (Y.F.); (X.-T.D.); (L.-J.W.); (E.-Y.J.); (P.N.V.);2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Shandong Energy Institute, Qingdao 266101, China;4.Qingdao New Energy Shandong Laboratory, Qingdao 266101, China |
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| Abstract: | Fucoxanthin, which is widely found in seaweeds and diatoms, has many benefits to human health, such as anti-diabetes, anti-obesity, and anti-inflammatory physiological activities. However, the low content of fucoxanthin in brown algae and diatoms limits the commercialization of this product. In this study, we introduced an excitation light at 488 nm to analyze the emitted fluorescence of Phaeodactylum tricornutum, a diatom model organism rich in fucoxanthin. We observed a unique spectrum peak at 710 nm and found a linear correlation between fucoxanthin content and the mean fluorescence intensity. We subsequently used flow cytometry to screen high-fucoxanthin-content mutants created by heavy ion irradiation. After 20 days of cultivation, the fucoxanthin content of sorted cells was 25.5% higher than in the wild type. This method provides an efficient, rapid, and high-throughput approach to screen fucoxanthin-overproducing mutants. |
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| Keywords: | flow cytometry fucoxanthin heavy ion irradiation Phaeodactylum tricornutum |
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