The effect of liposomes composed of phosphatidylserine and cholesterol on fertility rates using frozen thawed equine spermatozoa |
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| Authors: | D.J. Denniston J.K. Graham E.L. Squires S.P. Brinsko |
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| Affiliation: | 1. Programa de Ganadería, Instituto de Recursos Genéticos y Productividad Colegio de Postgraduados, Km 36.5, Montecillo, México-Texcoco, Estado de México C.P. 56230, Mexico;2. Ingeniería en Agrotecnología, Universidad Politécnica de Francisco I Madero, Tepatepec, Hidalgo, Mexico;3. Centro de Enseñanza, Investigación y Extensión en Producción Ovina, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico;4. Asesor Independiente, Mexico;5. Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuarias, UAEH, Mexico;6. Laboratorio de Biología de la Reproducción, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico;1. Drug Discovery Informatics Lab, QRC-Qasemi Research Center, Al-Qasemi Academic College, Baka El-Garbiah, Israel;2. Oral and Maxillofacial Surgery Department, Bone Regeneration Lab, Galilee Medical Center, Nahariya, Israel;3. Faculty of Medicine in the Galilee, Bar-ilan University, Ramat Gan, Israel;1. Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, USA;2. Veterinary Genetics Laboratory, University of California, Davis, CA, USA;3. Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, USA |
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| Abstract: | The objective of this study was to determine if the addition of liposomes composed of phosphatidylserine (PS) and cholesterol (CH) to equine sperm would improve pregnancy rates after sperm were cryopreserved. Ejaculates from four stallions, collected every other day during May and June were split, treated with PSCH liposomes or HBS (Hepes Buffered Saline) and cryopreserved. Fifty-two mares were bred over eighty estrous cycles with the frozen-thawed semen. The one cycle pregnancy rates of the mares inseminated with semen treated with liposomes (45%) were similar to mares inseminated with control semen (48%; p>0.05). Thus, at least at the levels used, PSCH liposomes added to equine sperm did not improve pregnancy rates of mares inseminated with cryopreserved semen. |
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| Keywords: | Cryopreservation liposomes phosphatidylserine equine |
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