RAPD Analysis of M_1 Generation of Gladiolus hybridus Hort Treated by EMS |
| |
Authors: | WANG Jingang GUO Ying CHE Daidi LIU Shenkui YANG Chuanpin |
| |
Affiliation: | 1. College of Horticulture, Northeast Agricultural University, Harbin 150030, China;Key Laboratory of Heredity Breeding, Northeast Forestry University, Harbin 150040, China 2. Departement of Foreign Language, Harbin Normal University, Harbin 150080, China 3. College of Horticulture, Northeast Agricultural University, Harbin 150030, China 4. Key Laboratory of Heredity Breeding, Northeast Forestry University, Harbin 150040, China |
| |
Abstract: | Gladiolus hybridus Hort is one of the world's famous cutting flowers. It is very popular because of the big size, bright color, various shape, and long period of bloom. New species should be cultivated in order to meet the consumers' need of asking for the new. Among the technologies of cultivating new species of flowers, mutagenic breeding is a shortcut. This study treated corm bud of G. hybridus Hort with EMS of different consistency. Then M1 after treated was analyzed by RAPD. The result showed that EMS was a very effective mutagenic agent for the corm bud of G. hybridus Hort. With the increase of consistency, the mutagenic range increased first, then decreased, among which 0.6% EMS treatment had the biggest influence. However, with the same EMS consistency, there was not close relevancy between the amount of mutagenic agent and the divergence of plant's genomes, which offered a molecular basis for selecting plants with good mutation. |
| |
Keywords: | Gladiolus hybridus Hort EMS RAPD |
本文献已被 CNKI 维普 万方数据 等数据库收录! |
|