| 黑龙江省黑木耳9个栽培菌株遗传多样性的RAPD分析 |
| |
| 引用本文： | 李玲,李杰,邹丽,白素英,牛黎明,马玉堃.黑龙江省黑木耳9个栽培菌株遗传多样性的RAPD分析[J].林业研究,2007,18(2):136-138. |
| |
| 作者姓名： | 李玲 李杰 邹丽 白素英 牛黎明 马玉堃 |
| |
| 作者单位： | 东北林业大学 哈尔滨150040 |
| |
| 基金项目： | The research was supported by Science Foundation of Northeast Forestry University （2004） |
| |
| 摘 要： | 本文对黑龙江省9个黑木耳 （Auricularia auricular）栽培菌株进行PCR-RAPD扩增和群体聚类分析, 在40个10bp随机引物中,筛选出13个引物.结果表明：黑木耳不同栽培菌株之间存在着丰富的多态性（96.05%）.用引物27和引物46的RAPD扩增指纹能有效分辨9个不同菌株的基因型,这一结果有助于在早期对黑木耳栽培菌株进行快速鉴别,为规范鉴别食用菌菌种混乱的局面提供科学依据.
|
| 关 键 词： | 黑木耳 RAPD分析 DNA指纹图谱 遗传多态性 |
| 文章编号： | 1007-662X（2007）02-0136-03 |
| 收稿时间： | 17 November 2006 |
| 修稿时间： | 2006-11-172006-12-19 |
RAPD analysis of genetic diversity of nine strains of <Emphasis Type="Italic">Auricularia auricular</Emphasis> cultivated in Heilongjiang Province |
| |
| Li?Ling,Li?Jie,Zou?Li,Bai?Su-ying,Niu?Li-ming,Ma?Yu-kun.RAPD analysis of genetic diversity of nine strains of Auricularia auricular cultivated in Heilongjiang Province[J].Journal of Forestry Research,2007,18(2):136-138. |
| |
| Authors: | Li Ling Li Jie Zou Li Bai Su-ying Niu Li-ming Ma Yu-kun |
| |
| Institution: | Northeast Forestry University, Harbin 150040, P. R. China;Northeast Forestry University, Harbin 150040, P. R. China;Northeast Forestry University, Harbin 150040, P. R. China;Northeast Forestry University, Harbin 150040, P. R. China;Northeast Forestry University, Harbin 150040, P. R. China;Northeast Forestry University, Harbin 150040, P. R. China |
| |
| Abstract: | Polymorphism of nine strains (CF05, CF09, 29, 916, AU9, Chang10, Chang7, 8808 and AU. Japanese) of A. auricular cultivated in Heilongjiang Province were analyzed by RAPD (Random Amplication polymorphic DNA). Thirteen primers were selected from forty PCR primers with 10bp long random primer. The results showed that nine strains of A. auricular have a high level of genetic diversity and the percentage of DNA polymorphic was 96.05. The genotypes of 9 strains of Auricularia auricular were identified by the fingerprints from primer 27 and primer 46 by RAPD analysis. The results are helpful for quickly identifying strains of A. auricular in its early breeding time, and also provides a powerful theoretic basis to differentiate strains (Auricularia auricular) whose morphology is very similar in breeding programs of edible fungus. |
| |
| Keywords: | Auricularia auricular RAPD analysis DNA fingerprint Genetic diversity |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
|
