| 豆大蓟马钠离子通道基因克隆及分析 |
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| 引用本文： | 潘雪莲,杨磊,袁琳琳,陈龙威,吴少英.豆大蓟马钠离子通道基因克隆及分析[J].热带生物学报,2022,13(3):249-258. |
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| 作者姓名： | 潘雪莲 杨磊 袁琳琳 陈龙威 吴少英 |
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| 作者单位： | 1.海南大学 三亚南繁研究院，海南 三亚 572024 |
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| 基金项目： | 海南省重点研发项目;国家自然科学基金 |
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| 摘 要： | 拟除虫菊酯类药剂的作用靶标为昆虫钠离子通道Nav，为了探明豆大蓟马（Megalurothrips usitatus）钠离子通道基因（MuNav）的特性，本研究采用PCR技术克隆得到MuNav序列（序列号MZ043856），该基因全长6 279 bp，编码2 093 aa，具有4个同源结构域，每个同源结构域含6个跨膜片段。同源比对发现，豆大蓟马与棕榈蓟马(Thrips palmi )Nav序列相似度高达94.88%，表明它们具有较近的亲缘关系。
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| 关 键 词： | 豆大蓟马 钠离子通道 基因克隆 序列分析 |
| 收稿时间： | 2022-01-15 |
Cloning and analysis of sodium channel of Megalurothrips usitatus |
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| PAN Xuelian,YANG Lei,YUAN Linlin,CHEN Weilong,WU Shaoying.Cloning and analysis of sodium channel of Megalurothrips usitatus[J].Journal of Tropical Biology,2022,13(3):249-258. |
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| Authors: | PAN Xuelian YANG Lei YUAN Linlin CHEN Weilong WU Shaoying |
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| Institution: | 1.Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 5720242.College of Plant Protection, Hainan University, Haikou, Hainan 5702283.Yazhou Bay Science and Technology Town, Yazhou, Sanya, Hainan 572024, China |
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| Abstract: | The strong concealment and difficult control of Megalurothrips usitatus lead to the extensive use of pyrethroids, which results in continuously increase in pesiticidal resistance of M. usitatus population in the field. The target of pyrethroid to pest insects is the sodium channel. Therefore, it is of great significance to explore the characteristics of M. usitatus sodium channel (MuNav) and analyze its resistance molecular mechanism. MuNav (Accession number: MZ043856) was cloned by PCR with a full-length of 6 279 bp, which encodes 2093aa. MuNav is embedded with four homologous domains containing six transmembrane fragments. Homologous comparison showed that the similarity of sodium channel between M. usitatus and Thrips palmi was 94.88%, indicating their close relationship. |
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