| 鹅细小病毒广西株的分离鉴定 |
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| 引用本文： | 秦顺华,覃芳芸,颜健华,潘杰,胡丽萍,杨荣,付薇,熊毅.鹅细小病毒广西株的分离鉴定[J].动物医学进展,2009,30(5). |
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| 作者姓名： | 秦顺华 覃芳芸 颜健华 潘杰 胡丽萍 杨荣 付薇 熊毅 |
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| 作者单位： | 1. 广西兴安县动物疫病预防控制中心,广西兴安,541300
2. 广西区动物疫病预防控制中心,广西南宁,530001 |
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| 摘 要： | 将广西某养鹅场5日龄发病的雏鹅肝脾处理后接种于10日龄的鹅胚,分离到一株病毒,对死胚收集的尿囊液进行荧光PCR确诊后,对其VP3基因进行扩增测序,通过与GDFSH株(Guangdong)、82-0308株(Taiwan)、HLJ01株(Heilongjiang)和HBZF07株(Hebei)VP3基因序列分析,核苷酸序列同源性在96.2%以上,从分子水平上鉴定该分离株是鹅细小病毒.
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| 关 键 词： | 鹅细小病毒 分离 鉴定 |
Isolation and Identification of Goose Parvovirus GX1 Strtain |
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| QIN Shun-hua,QIN Fang-yun,YAN Jian-hua,PAN Jie,HU Li-ping,YANG Rong,FU Wei,XIONG Yi.Isolation and Identification of Goose Parvovirus GX1 Strtain[J].Progress In Veterinary Medicine,2009,30(5). |
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| Authors: | QIN Shun-hua QIN Fang-yun YAN Jian-hua PAN Jie HU Li-ping YANG Rong FU Wei XIONG Yi |
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| Institution: | 1.Guangxi Xingan Center for Animal Disease Control and Prevention;Xingan;Guangxi;541300;China;2.Guangxi Center for Animal Disease Control and Prevention;Nanning;530001;China |
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| Abstract: | With 10-days goose embryo,a strain of virus was isolated from the spleen and liver of 5 days old gosling died from gosling plague in Guangxi.It was determined by the real-time quantitative PCR method.The length of 589 nucleotides of VP3 were amplified,then the fragments were cloned and sequenced.Analysing the sequences of VP3 gene of the isolated strain,GDFSH strain,82-0308 strain,HLJ01 strain and HBZF07 strain,their homologies of nucleotides were 96.2% to 98%.The results showed that the isolated strain was... |
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| Keywords: | Goose parvovirus isolation identification |
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