| 报春石斛再生体系的建立 |
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| 引用本文： | 张莹,王雁,李振坚.报春石斛再生体系的建立[J].浙江林学院学报,2009,26(4):603-606. |
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| 作者姓名： | 张莹 王雁 李振坚 |
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| 作者单位： | 1. 中国林业科学研究院,林业研究所,北京,100091;中国林业科学研究院,亚热带林业研究所,浙江,富阳,311400
2. 中国林业科学研究院,林业研究所,北京,100091 |
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| 基金项目： | 国家林业局引进国际先进农业科学技术计划(948计划)项目，中央级公益性科研院所基本科研业务费专项资金项目 |
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| 摘 要： | 以茎段为外植体，建立了报春石斛Dendrobium primulinum的离体培养再生体系。结果表明：愈合组织诱导以培养基MS（Murashige and Skoog）＋5．0mg·L^-16-苄氨基嘌呤（6-BA）＋1．5mg·L^-1 2，4-D为最优，诱导率达33.33％；分化和增殖培养基均以MS＋1．0mg·L^-1 6-BA＋0．1mg·L^-1萘乙酸（NAA）＋体积分数10％椰子水为佳，分化率最高可达8320％，增殖倍数达3．82．外源物质椰子水可促进不定芽的形成及增殖。形成的无根苗转移到1／2MS＋0．5mg·L^-1 6-BA＋1．0mg·L^-1NAA＋0．5g·L^-1活性炭培养基上诱导生根发育形成完整植株。图1表4参12
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| 关 键 词： | 植物学 报春石斛 茎段 再生体系 |
Regeneration system for Dendrobium primulinum |
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| ZHANG Ying,WANG Yan,LI Zhen-jian.Regeneration system for Dendrobium primulinum[J].Journal of Zhejiang Forestry College,2009,26(4):603-606. |
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| Authors: | ZHANG Ying WANG Yan LI Zhen-jian |
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| Institution: | ZHANG Ying, WANG Yan, LI Zhen-jian (1. Research Institute of Forestry, The Chinese Academy of Forestry, Beijing 100091, China; 2. Research Institute of Subtropical Forestry, The Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China) |
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| Abstract: | An in vitro plant regeneration system ofDendrobium primulinum was established using stem sections as explants. Results showed that the optimum callus inducing medium was Murashige and Skoog （MS） ＋ 5.0 mg.L^-1 6-BA ＋ 1.5 mg.L^-1 2, 4-D with an induction rate of 33.3%. Adventitious buds were effectively generated and proliferated on MS ＋ 1.0 mg.L^-16-BA ＋ 0.1 mg.L^-1 NAA ＋ coconut water 10% with a differentiation rate of 83.2% and proliferation of 3.82 times. Exogenous coconut water promoted adventitious bud generation and proliferation. The best rooting medium was 1/2MS ＋ 0.5 mg.L^-1 6-BA ＋1.0 mg.L^-1NAA ＋ 0.5 g.L^-1 activated carbon. Ch, 1 fig. 4 tab. 12 ref.] |
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| Keywords: | botany Dendrobium primulinum stem sections regeneration system |
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