不同海拔高度和竹龄对黄甜竹叶精油成分及其生物活性的影响

为探究海拔和竹龄对黄甜竹叶精油品质的影响，本研究采用气相色谱-质谱联用技术（GC-MS）测定不同海拔高度下新叶和老叶精油的化学成分，并分别采用平板抑菌法和1,1-二苯基-2-三硝基苯肼（DPPH）法测定各竹叶精油的抑菌效果和抗氧化活性。结果表明，黄甜竹叶精油中的成分以烷类、醇类、萜烯类和芳香族化合物为主，中高海拔有利于萜烯类化合物的合成和积累，新叶精油中的醇类、萜烯类和芳香族化合物含量高于老叶。各竹叶精油对干腐病菌（Fusarium）和葡萄座腔菌（Botryosphaeria dothidea）均有抑制效果，海拔越高竹叶的抑菌效果越好，新叶精油的抑菌效果强于老叶。各竹叶精油均表现出较高的抗氧化活性，表现为低海拔>中高海拔、新叶>老叶。本研究结果为黄甜竹叶资源的开发提供了数据支撑和新的利用方向。

Effects of Different Altitudes and Bamboo Ages on Essential Oil Composition and Its Bioactivity of Acidosasa edulis Leaves

In order to study the effect of altitudes and bamboo ages on the essential oils quality of Acidosasa edulis leaves， the chemical composition of the essential oils of new and old leaves at different altitudes was determined by gas chromatography-mass spectrometry （GC-MS）. The disk-diffusion and DPPH method were used to measure the antibacterial and antioxidant activity of bamboo leaves essential oils. The results showed that the composition of the essential oils of Acidosasa edulis leaves was dominated by alkanes， alcohols， terpenoids and aromatic compounds.The medium to high altitudes were good for the synthesis and accumulation of terpenoids. The content of alcohols， terpenoids and aromatic compounds in the new leaves essential oils was higher than that of old. Bamboo leaves essential oils showed inhibitory effects on both Fusarium and Botryosphaeria dothidea， the higher the altitudes the better the inhibitory effects， and the new leaves essential oils showed stronger inhibitory effects than old. Bamboo leaves essential oils showed high antioxidant activity.The antioxidant capacity of the essential oils was stronger at low altitudes than at middle and high altitudes， and the new leaves was stronger than old. This study results provides data support and new utilization directions for the development of Acidosasa edulis leaves resources.