不同施肥处理对杨树林地土壤微生物群落的影响

土壤微生物多样性是提高人工林土壤生产力的重要因素之一，由于杨树二代林土壤退化严重，为了提高退化土地的土壤微生物多样性，找出合适的肥料配比方案，以安徽北方5年生杨树二代林为研究对象，进行连续4年的施肥试验后，采集土壤样品，采用高通量测序技术分析不同施肥处理杨树林地土壤微生物群落变化特征，并与环境因子进行RDA分析。结果表明：杨树二代林中，占优势细菌属的有MND1、Gaiella、小梨形菌属(Pirellula)及RB41，土壤表层施肥和未施肥土壤细菌菌属差异显著。蜡蚧菌属(Lecanicillium)、被孢霉属(Mortierella)及镰刀菌属(Fusarium) 3种菌属为真菌优势菌属，真菌群落在土层中分布相关性较小。施用氮肥250 g/株、磷肥500 g/株、有机肥500 g/株及土壤改良生物菌肥200 g/株和氮肥125 g/株、磷肥500 g/株、钾肥75 g/株及土壤改良生物菌肥100 g/株其表层土壤的细菌分布较均匀且多样性较高，这两种施肥方案适合改善林地表层土壤细菌群落多样性。施用氮肥500 g/株、钾肥75 g/株、有机肥500 g/株及土壤改良生物菌肥150 g/株和...

Effects of Different Fertilization Treatments on Soil Microbial Communities in Poplar Forest Land

Soil microbial diversity is one of the important factors to improve soil productivity in planted forests. Due to the serious soil degradation of the second-generation Poplar forest and with the aim of improving the soil microbial diversity of degraded land and finding out the suitable ratio of fertilizer solution, 5-year-old second-generation Poplar forests in northern Anhui were used as the study objects. After 4 consecutive years of fertilization experiment, soil samples were collected, and high-throughput sequencing technology was used to analyze the change characteristics of soil microbial communities in Poplar forests under different fertilization treatments with an RDA analysis of environmental factors. The results showed that the MND1, Gaiella, Pirellula and RB41 were the dominant bacterial genera in the second-generation Poplar forest, and there were significant differences of bacterial genus between fertilized soil surface and unfertilized soil. Lecanicillium, Mortierella and Fusarium were 3 dominant fungal genera and the distribution of fungal communities in the soil layer was less relevant. Application of nitrogen fertilizer 250 g/plant, phosphate fertilizer 500 g/plant, organic fertilizer 500 g/plant and soil improvement biological bacterial fertilizer 200 g/plant and application of nitrogen fertilizer 125 g/plant, phosphate fertilizer 500 g/plant, potash fertilizer 75 g/plant and soil improvement biological bacterial fertilizer 100 g/plant had a more uniform distribution and higher diversity of bacteria in the surface soil, and were suitable for improving the diversity of forest surface soil bacterial community. Application of nitrogen fertilizer 500 g/plant, potash fertilizer 75 g/plant, organic fertilizer 500 g/plant and soil improvement biological bacterial fertilizer 150 g/plant and application of nitrogen fertilizer 250 g/plant, phosphate fertilizer 750 g/plant, potash fertilizer 25 g/plant and soil improvement biological bacterial fertilizer 150 g/plant had a more uniform distribution of species and a higher diversity of fungi in the deep soil, and were suitable for improving the diversity of forest deep soil fungal community. Urease had a great influence on the community composition of soil bacteria and fungi, and can be an important soil environmental factor in Poplar forest land.