不同载体菌肥对刺槐光合特性及土壤养分、细菌群落的影响

为探究接种粘质沙雷氏菌（Serratia marcescens N1.14）X-45和不同载体基质固态发酵制备而成的菌肥的应用效果，并以此为结果筛选出可有效替代珍稀资源泥炭发挥肥效的载体，本研究设置7个处理：未施肥对照组（CK）、泥炭对照组（T0）、秸秆组（T1）、麦麸组（T2）、秸秆∶麦麸=1∶1组（T3）、秸秆∶麦麸∶棉籽壳=1∶1∶1组（T4）、秸秆∶麦麸∶豆饼=1∶1∶1组（T5），通过盆栽试验测定不同菌肥施用下刺槐（Robinia pseudoacacia L.）的光合特性参数和土壤养分含量，采集效果较好菌肥处理下的根际土样本进行16S rDNA高通量测序。结果表明，与泥炭对照相比，除单一载体秸秆外，其余载体处理均可不同程度地改善刺槐光合特性及土壤营养环境，其中以T3、T4应用效果最显著，光合特性参数和土壤养分含量提升明显。基于16S rDNA高通量测序的刺槐根际土微生物多样性结果显示，菌肥的施入可显著改善土壤菌群结构和组成，提升益生菌群Actinomadura和Mesorhizobium的相对丰度，同时其丰度与水解氮、有效磷含量呈正相关，与pH值、速效钾含量呈负相关，即土壤...

Effects of Different Carrier Bacterial Fertilizers on Photosynthetic Characteristics,Soil Nutrients and Bacterial Communities of Robinia pseudoacacia L.

This study was to explore the application effect of bacterial fertilizer prepared by inoculating Serratia marcescensN1.14 X-45 and solid-state fermentation of different carrier matrices， at the same time， select the treatment that can effectively replace peat carrier to play a fertilizer effect. Seven treatments were set as unfertilized （CK）， peat （T0）， straw （T1）， wheat bran （T2）， straw∶wheat bran =1∶1 （T3）， straw∶wheat bran∶cottonseed shell =1∶1∶1(T4) and straw∶wheat bran∶soybean cake =1∶1∶1 (T5). A pot experiment was conducted to determine the photosynthetic characteristics parameters and soil nutrient content of Robinia pseudoacacia L. under different bacterial fertilizers and collect rhizosphere soil samples with good effect under bacterial fertilizer treatment for 16S rDNA high-throughput sequencing. Results showed that compared with peat, the photosynthetic characteristics and soil nutrient environment of Robinia pseudoacacia were improved by all the carrier treatments except straw. Among them, T3 and T4 had the most significant application effect, and the photosynthetic characteristic parameters and soil nutrient content increased significantly. Based on 16S rDNA high-throughput sequencing, the results showed that the application of bacterial fertilizer could significantly improve the structure and composition of soil microbial community, at the same time, increase the relative abundance of Actinomadura and Mesorhizobium. Its abundance was positively correlated with hydrolyzed nitrogen and available phosphorus content, and negatively correlated with pH and available potassium content. That is, the increase in the content of hydrolyzed nitrogen and available phosphorus in the soil promoted the increase in the relative abundance of Actinomadura and Mesorhizobium. This study provides theoretical basis and data support for the development and utilization of microbial fertilizers and the improvement of inefficient forests.