2019-2020山东小麦区试品系55个基因的等位基因分布

为分析2019—2020年山东省小麦区试参试品系携带优异等位基因情况,利用产量、品质、抗性、开花期等55个基因的64个竞争性等位基因特异性聚合酶链式反应（KASP）标记对126份参试品系进行分子标记检测。结果表明,90%以上KASP标记具有较好的分型结果,可高效地进行基因型鉴定。20个基因的优异等位基因频率高于80%,包括Vrn-A1、Vrn-B1、Vrn-D1、Ppd-A1、Ppd-B1、Ppd-D1、Psy-D1、Glu-A3g、Rht-D1、Pinb-D1、TaCwi-A1-1、TaGW2-6B、TaSus1-7B、TaGASR7-A1、1-feh-w3、TaDreb-B1、PRA1、PRR-B1、TaFT3-B1和TaMOT1-D1;26个基因的优异等位基因频率低于30%,包括Vp1-B1、Ppo-D1、TaPds-B1、Zds-A1、TEF-7A、Lr46、Glu-B3g、TaGS5-A1、TaCwi-4A、1B/1R、Rht-B1、Lr68、TaELF3-B1、Pina-D1、Sbwm1、TaPHS1、Pm21、COMT-3B、TaCKX-D1、TaSdr-B1、Pch1、...

Allele Distribution of 55 Genes in Wheat Lines Participating in 2019-2020 Shandong Trial

To analyze gene distribution in wheat lines involved in 2019—2020 Shandong regional trial, one hundred and twenty-six wheat lines were tested with 64 Kompetitive Allele Specific polymerase chain reaction （KASP） markers which were specific to 55 genetic loci. The markers are related to yield, quality, resistance, flower time and etc. Over 90% KASP markers give acceptable genotyping, which provides an efficient technology for gene identification. Favorable allele frequencies of 20 genes are above 80%, including Vrn-A1, Vrn-B1, Vrn-D1, Ppd-A1, Ppd-B1, Ppd-D1, Psy-D1, Glu-A3g, Rht-D1, Pinb-D1, TaCwi-A1-1, TaGW2-6B, TaSus1-7B, TaGASR7-A1, 1-feh-w3, TaDreb-B1, PRR-A1, PRR-B1, TaFT3-B1 and TaMOT1-D1. Favorable allele frequencies of 26 genes are below 30%, including Vp1-B1, Ppo-D1, TaPds-B1, Zds-A1, TEF-7A, Lr46, Glu-B3g, TaGS5-A1, TaCwi-4A, 1B/1R, Rht-B1, Lr68, TaELF3-B1, Pina-D1, Sbwm1, TaPHS1, Pm21, COMT-3B, TaCKX-D1, TaSdr-B1, Pch1, Lr34, Yr15, Fhb1, TaMoc-7A and TaGS-D1, notably,favorable alleles of TaMoc-7A, Lr34, Fhb1, Pch1, Yr15, and TaSdr-B1 are not detected. Favorable allele frequencies of 9 genes are between 30% and 80%, including Pinb2-V, TaPod-A1, Lox-B1, Glu-A1, Glu-D1, TaGS2-B1, TaGW2-6A, TaSus1-7A, and Lr14a. Favorable allele frequencies of 55 genes show polarization trend. Among all tested lines, JinongCH03, CG086, and JinongCH01 are the only one possessing favorable alleles of TaCKX-D1, Pm21, and COMT-3B, which could be used as gene donors for kernel weight, powdery mildew resistance, and stem lignin content improvement and breeding. In this study, genotypes of 55 genes in 126 wheat lines are clarified, allelic distribution of important agronomic genes in wheat lines attending the Shandong regional trial was basically clarified, which will serve as the foundation for marker assistant selection breeding.