冬瓜果肉叶绿素含量遗传分析

对冬瓜果肉叶绿素含量遗传规律进行分析，以期为冬瓜果肉颜色控制基因的挖掘和果肉颜色的改良育种奠定基础。以冬瓜果肉白色纯化自交系(LT-1)为P₁，果肉绿色纯化自交系(LT-2)为P₂，构建四代遗传群体(P₁、P₂、F₁、F₂)，利用主基因+多基因混合模型的遗传分析方法，通过分析主基因和多基因对冬瓜果肉种的叶绿素含量影响，探究冬瓜果肉叶绿素含量的遗传规律。结果表明，冬瓜果肉中叶绿素含量是由2对加性—显性—上位性主基因+加性显性多基因遗传模型调控，F₂分离群体中的主效基因遗传率为82.0696%，第一对主基因的加性效应d_a为-1.477，显性效应h_a为-1.465，且显性度h_a/d_a接近1，第二对主基因的加性效应d_b和显性效应h_b分别为-0.835、-0.715，显性度h_a/d_a...

Genetic Analysis of Chlorophyll Content in the Flesh of Wax Gourd

The paper aims to explore the genetic law of chlorophyll content in wax gourd flesh and lay a foundation for improving wax gourd breeding concerning flesh color regulating gene. Genetic groups P₁, P₂, F₁ and F₂ were obtained by using white flesh purified inbred line (LT-1) as P₁ and green flesh purified inbred line (LT-2) as P₂. Using the genetic analysis method of main gene plus polygene mixed model, the genetic law of chlorophyll content in wax gourd flesh was explored by analyzing the effects of main gene and polygene on chlorophyll content of wax gourd flesh. The results showed that the chlorophyll content in wax gourd flesh was regulated by two pairs of additive- dominant- epistatic major genes plus additive dominant polygenic genetic model. In F₂ population, the heritability of the main gene was 82.0696%. The additive effect d_a of the first pair of major genes was -1.477, and the dominant effect h_a was -1.465, and the dominance degree h_a/d_a was close to 1. The second additive effect on major genes d_b was -0.835 and the dominant effect h_b was -0.715, and the dominance degree h_a/d_a was close to 1. It showed that additive effect and dominant effect were equally important for genetic control of chlorophyll content in the same pair of main genes. The additive and dominant effects of the two pairs of major genes were negative, which played a reducing effect. Therefore, to increase chlorophyll content in F₂ generation, we should pay attention to the genetic effect of main genes, and use additive effect and dominant effect at the same time.