丙酸睾酮对三疣梭子蟹氨基酸和脂肪酸组成的影响

为研究丙酸睾酮(TP)对三疣梭子蟹氨基酸和脂肪酸组成的影响，试验以三疣梭子蟹I期幼蟹为材料，随机分为对照组(饲料中无丙酸睾酮)、TP 200处理组(饲料含200 mg·kg^-1TP)和TP 400处理组(饲料含400 mg·kg^-1TP)，养殖90 d后，采用液相色谱质谱联用法检测血清TP、睾酮(T)和雌二醇(E₂)含量，并测定各组生长指标及存活率，检测肌肉和肝胰腺的基本营养成分、氨基酸及脂肪酸组成。结果显示，三疣梭子蟹血清中性激素含量较高，表明其具有较强的性激素富集能力。添加TP对三疣梭子蟹肝胰腺指数及蜕壳频率无显著影响，但成活率显著降低(P<0.05),TP 200组三疣梭子蟹的增重率、特定生长率及全甲宽等指标显著高于对照组及TP 400组(P<0.05)。TP 200组和TP 400组的总氨基酸、必需氨基酸含量与对照组相比无显著差异，但精氨酸含量显著降低(P<0.05)。TP 200组和TP 400组肌肉脂肪酸组成和含量与对照组相比无显著差异，但肝胰腺中部分脂肪酸特别是高度不饱和脂肪酸(C20∶2...

Effects of Testosterone Propionate on Amino Acid and Fatty Acid Composition of Portunus trituberculatus

In order to investigate the effects of testosterone propionate on the composition of amino acids and fatty acids of swimming crab （Portunus trituberculatus）， the juvenile crab of stage I was randomly divided into control group （no testosterone propionate （TP） in the feed）， TP 200 group （200 mg·kg^-1 TP in the feed） and TP 400 group （400 mg·kg^-1 TP in the feed）. After rearing for 90 days， the contents of TP， testosterone （T） and estradiol （E₂） in serum of swimming crab were determined by liquid chromatography-mass spectrometry （LC-MS）； the growth index and survival rate were recorded； basic nutrient composition， amino acid and fatty acid composition of muscle and hepatopancreas were analyzed for comparison. The results showed that the serum of P. trituberculatus contained a high content of sex hormones， indicating that it has a strong ability to enrich sex hormones. TP had no significant effect on the hepatopancreatic index or molting frequency of P. trituberculatus， but significantly reduced the survival rate （P<0.05）. The weight gain rate， specific growth rate and full carapace width of P. trituberculatus in TP 200 group were significantly higher than those in control group and TP 400 group （P<0.05）. The contents of total amino acids and essential amino acids in muscle and hepatopancreas of TP 200 and TP 400 groups were not significantly different from those of the control group. However， the content of arginine decreased significantly； there was no significant difference in the composition and content of fatty acids in the muscle between TP 200/400 groups and control group， but the content of some fatty acids in hepatopancreas， especially highly unsaturated fatty acids （C_20∶2， EPA and DHA） decreased significantly in the experimental groups， which may be related to the acceleration of fatty acid oxidation and energy supply by sex hormones. These results would provide basic data for the study of the effects of exogenous sex hormones on the metabolism of amino acids and fatty acids in invertebrates.