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二次发酵提取茶皂素的方法研究
引用本文:王文杰,陈长庚,张必桦,黄建琴,吴新荣,徐奕鼎,方吴云,王烨军,雷攀登,吴琼.二次发酵提取茶皂素的方法研究[J].中国农学通报,2014,30(30):296-301.
作者姓名:王文杰  陈长庚  张必桦  黄建琴  吴新荣  徐奕鼎  方吴云  王烨军  雷攀登  吴琼
作者单位:安徽省农业科学院茶叶研究所,,,安徽省农业科学院茶叶研究所,,安徽省农业科学院茶叶研究所,,,安徽省农业科学院茶叶研究所,安徽省农业科学院茶叶研究所
基金项目:国家星火计划子课题“标准茶园高效栽培关键技术集成与应用”(2012GA710001);安徽省农科院创新团队建设项目“茶树种质资源创新与 利用”(11C0809);安徽省农科院农业科技创新项目“茶皂素对茶尺蠖毒性作用机理研究”(13B0838)
摘    要:为提取饼粕中的茶皂素,根据饼粕中含有较多糖类物质的特点,对饼粕进行酒精发酵研究。结果表明:采用二次发酵法,可以成功地促进饼粕的酒精发酵,每百克饼粕因酒精发酵而被消耗12.58 g 糖类物质;茶皂素的提取率达80%左右,且粗提茶皂素纯度高,可以达到62.37%;玉米粉与饼粕的配比为1:1.5较为合适;浓缩能耗低,初提液茶皂素浓度可达78.26 mg/mL。

关 键 词:水质浑浊度  水质浑浊度  
收稿时间:2014/5/21 0:00:00
修稿时间:2014/5/21 0:00:00

Method of Abstracting Tea-saponin with Secondany Fermemtation
Wang Wenjie,Chen Changgeng,Zhang Bihua,Huang Jianqin,Wu Xinron Xu Yiding,Fang Wuyun,Wang Yejun,Lei Pandeng,Wu Qiong.Method of Abstracting Tea-saponin with Secondany Fermemtation[J].Chinese Agricultural Science Bulletin,2014,30(30):296-301.
Authors:Wang Wenjie  Chen Changgeng  Zhang Bihua  Huang Jianqin  Wu Xinron Xu Yiding  Fang Wuyun  Wang Yejun  Lei Pandeng  Wu Qiong
Institution:(Tea Research Institute, Anhui Academy of Agricultural Sciences, Qimen Anhui 245600)
Abstract:Alcohol fermentation was carried out to extract tea-saponin based on the feature that there are a lot of saccharides substances in tea cake. The result showed that secondary fermentation could successfully promote alcohol fermentation. 12.58 g carbohydrate per 100 g cake was consumed during alcohol fermentation. The extraction percentage could reach 80%, and the purity of crude extraction could achieve 62.37%. It was appropriate when the proportioning of corn meal and cake was 1:1.5. The energy consumption of concentration was low. The concentration of tea-saponin in original extracts was 78.26 mg/mL.
Keywords:tea-saponin  secondary fermentation  extraction percentage  proportioning of corn meal
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