乌拉尔甘草毛状根的诱导及离体培养

为获得甘草的药用成分和保护甘草野生资源，利用发根农杆菌ATCC15834、A4 侵染乌拉尔甘草幼苗、子叶、下胚轴，获得毛状根，建立了乌拉尔甘草毛状根培养系统，经PCR和毛细管凝胶电泳检测，在248 bp 有rolA 基因，在490 bp 有rolC 基因，证实发根农杆菌基因片段在甘草毛状根的基因组中整合并得到表达，其中无菌实生苗毛状根诱导率达79.5%，毛状根分枝较多，生长速度快，在不添加激素的1/2MS液体培养基中生长明显，培养25 天后，鲜重平均增殖达19.13 倍，乌拉尔甘草毛状根培养体系的建立为进一步利用生物反应器规模化培养甘草药用活性成分提供了参考。

Induction and in Vitro Culture of Hairy Roots of Glycyrrhiza uralensis Fisch

To obtain medicinal components and protect the wild resource of Glycyrrhiza uralensis, two Agrobacterium rhizogenes ATCC15834 and A4 were used to induce the hairy root from cotyledons, hypocotyls and seedlings of Glycyrrhiza uralensis Fisch. Hairy root was obtained successfully and the system of hairy root induction was established. There were about 248 bp-rolA gene and 490 bp-rolC detected from the hairy-root by PCR and capillary gel electrophoresis which indicated that the gene segments of Agrobacterium rhizogenes had been integrated and expressed in hairy root of Glycyrrhiza uralensis Fisch. The hairy root induction rate was 79.5% by needle stabbing. The hairy roots grew faster and with much more branches in 1/2MS medium. When cultured for 25 days, the average fresh weight growth rate was 19.13 times. Establishment of hairy root culture system of Glycyrrhiza uralensis Fisch provided a foundation for the industrial production of active drug component in bioreactors.