| 木麻黄AFLP分析体系的建立及引物筛选 |
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| 引用本文： | 黄桂华,高丽丽,仲崇禄,苏晓华,张勇,陈羽,陈珍,谢冬勇. 木麻黄AFLP分析体系的建立及引物筛选[J]. 中南林业科技大学学报(自然科学版), 2007, 27(1): 20-24,34 |
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| 作者姓名： | 黄桂华 高丽丽 仲崇禄 苏晓华 张勇 陈羽 陈珍 谢冬勇 |
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| 作者单位： | 中国林业科学研究院热带林业研究所 广东广州510520(黄桂华，仲崇禄，张勇，陈羽，陈珍)，华南农业大学园艺学院 广东广州510642(高丽丽)，中国林业科学研究院林业研究所 北京100091(苏晓华)，广州市绿化公司 广东广州510170(谢冬勇) |
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| 基金项目： | 国家高技术研究发展计划(863计划);广东省自然科学基金;中国-澳大利亚合作项目;广东省科技计划 |
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| 摘 要： | 采用CTAB法改良技术提取木麻黄基因组DNA,在提取液中加入2%β-巯基乙醇防氧化和2%的PVP去除酚类等物质,获得高质量的基因组DNA,以满足AFLP对模板DNA高质量的要求;采用分步酶切和连接后,进行预扩和选扩,摸索建立和优化了木麻黄AFLP分析体系,探讨了木麻黄AFLP分析的影响因素;从64对AFLP核心引物组中筛选出了8对适合木麻黄分析的引物组合.同时探讨了AFLP技术在木麻黄研究领域的应用前景.
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| 关 键 词： | 生物技术 分析体系 引物 木麻黄 |
| 文章编号： | 1000-2502（2007）01-0020-05 |
| 收稿时间： | 2006-09-10 |
| 修稿时间： | 2006-09-10 |
Establishment of the Analytical System for Casuarinaceae AFLP and Its Primer Selection |
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| HUANG Gui-hua,GAO Li-li,ZHONG Chong-lu,SU Xiao-hua,ZHANG Yong,CHEN Yu,CHEN Zhen,XIE Dong-yong. Establishment of the Analytical System for Casuarinaceae AFLP and Its Primer Selection[J]. , 2007, 27(1): 20-24,34 |
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| Authors: | HUANG Gui-hua GAO Li-li ZHONG Chong-lu SU Xiao-hua ZHANG Yong CHEN Yu CHEN Zhen XIE Dong-yong |
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| Affiliation: | 1. Research Institute of Tropical Forestry, CAF, Guangzhou 510520, Guangdong, China; 2. South China Agricultural University, Guangzhou 510641, Guangdong, China; 3. Institute of Forestry Research, CAF, Beijing 100091, China; 4. Guangzhou Green Company, Guangzhou 510170, Guangdong, China |
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| Abstract: | An improved method of CTAB was used in this study to extract the total DNA of Casuarinaceae.Then,high quality genomic DNA was isolated for AFLP analysis with 2% of mercapto-ethanol in the extracting solution to prevent formation of oxides and 2% of PVP to eliminate phenols from genomic DNA.By means of double enzyme restriction,adapter-ligation,pre-amplification and selective amplification,AFLP analytical system of Casuarinaceae was established and optimized.8 primer combinations were selected from 64 pairs of core combinations of AFLP after a series of tests.The application prospect of AFLP in Casuarinaceae research field was also discussed. |
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| Keywords: | AFLP |
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