| 甘薯DNA的小量快速提取 |
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| 引用本文： | 张换样,李静,南芝润,焦改丽.甘薯DNA的小量快速提取[J].山西农业科学,2009,37(1):12-14. |
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| 作者姓名： | 张换样 李静 南芝润 焦改丽 |
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| 作者单位： | 山西省农业科学院棉花研究所,山西,运城,044000 |
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| 摘 要： | 以甘薯幼叶为试验材料,用小量快速法提取转基因甘薯总DNA。所提取的DNA以0.8%琼脂糖凝胶电泳检测和用试ECORI对DNA进行酶切。结果证明,该方法提取的DNA质量较高,可满足RAPD标记、PCR检测和Southernblot杂交等技术分析的需要。为甘薯及其他多糖类植物DNA的提取提供了一个所需材料量少、简便、快速的方法。
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| 关 键 词： | 甘薯 DNA提取 幼叶 简便快速 |
A Simple and Quick Extraction of Genomic DNA in Sweet Potato |
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| ZHANG Huan-yang,LI Jing,NAN Zhi-run,JIAO Gai-li.A Simple and Quick Extraction of Genomic DNA in Sweet Potato[J].Journal of Shanxi Agricultural Sciences,2009,37(1):12-14. |
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| Authors: | ZHANG Huan-yang LI Jing NAN Zhi-run JIAO Gai-li |
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| Institution: | ( Institute of Cotton Research, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China) |
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| Abstract: | Genomic DNA from transgenic sweet potato young leaves was extracted by a simple and quick extraction method. The method not only had simple and quick extracted speed but also would get pure and intact DNA. The extracted DNA could be cut by restrictive enzyme and could be used to PCR, Southern analysis. The method had displayed fine characteristics such as simple,quick, economical and effective. So it is a good way for us to extract genomic DNA of sweet potato and other plants. |
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| Keywords: | Sweet potato DNA extract Young leaves Simple and quick |
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