利用巢式PCR对海南槟榔（Areca catechu L.）黄化病的初步检测

槟榔黄化病是世界各地槟榔生产上普遍发生的毁灭性病害。至今没有根治的有效方法，根本原因是其病原还没有明确鉴定。有报道表明槟榔黄化病的病原可能是植原体。本文利用巢式PCR方法，对海南3个市县采集的28个黄化病植株的不同器官进行了植原体检测，结果表明在2市县9个槟榔黄化病病株叶片中检测到了植原体，而在根和茎中没有发现植原体。同源性聚类发现其属于翠菊黄花组B亚组。

Detection of the phytoplasmas associated with yellow leaf disease of Areca catechu L. in Hainan province of China by nested PCR

Yellow leaf disease （YLD） of the areca nut palm was a severe disease that occurred mainly in Southeast Asia.Although YLD has similar symptoms in different regions,it has been proven to be induced by different pathogens.In this work,28 samples of diseased palms were collected from three different counties of Hainan Province,China.Their 16S rDNA was studied by the nested PCR to detect the phytoplasma associated with YLD.The results showed that amplification was observed only in leaf samples,but not in stem or root samples,and phytoplasmas were detected only in 9 samples collected from 2 counties of Hainan pronvice.High boostrap values suggested that arecanut YLD phytoplasma is most closely related to Paulownia Witches ＇ Broom Phytoplasma and is a member of the Aster yellows group （group 16SrⅠ-B）.