Evaluation of serum iso-amylase in the normal dog using an improved electrophoretic technique |
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Authors: | J W Simpson D L Doxey G Keay |
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Institution: | (1) Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, Edinburgh, UK |
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Abstract: | Six iso-amylase fractions are known to exist in human serum, three originating from the salivary glands and three from the pancreas. Although it is known that a different number and source of iso-amylase fractions occur in the dog, the routine detection of all the canine iso-amylase fractions has not been previously established. Earlier methods detected either two iso-amylase fractions or, in a proportion of cases, four fractions. A method is described which detects four iso-amylase fractions in 95% of normal canine serum samples. Trials have shown the method is reproducible and that freezing at -40°C has no effect on iso-amylase activity. The normal values for iso-amylase are recorded in 18 normal dogs. |
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Keywords: | amylase dog electrophoresis iso-amylase serum |
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