影响“达赛莱克特”草莓茎尖培养因素的研究

以“达赛莱克特”草莓匍匐茎作为外植体，研究MS培养基浓度对初代培养试管苗成活率的影响．结果表明，将MS培养基浓度减半（1／2MS）可有效降低外植体褐化，成活率比MS培养基高，达71．8％，且试管苗生长良好．切取试管苗茎尖研究影响其成活的因素，结果发现茎尖培养的适宜培养基为MS＋6-BA0．5mg／L＋NAA0．01mg／L＋蔗糖30g／L；为获得脱毒苗，进行茎尖培养时茎尖以0．3mm为宜；“达赛莱克特”草莓试管苗在38℃下处理38d后切取茎尖0．5mm，既可脱毒又可提高茎尖成活率，是生产脱毒苗的有效方法．

Study of several factors affecting culture of shoot tip of strawberry 'Darselect' in Vitro

Effect of MS medium strength on survival of shoots in vitro during initial culture- was studied,make the＇ Darselect＇ as material. The results showed that the browning of ex- plants was inhibited and 71.8% of survival rate were obtained in 1/2 strength of MS medi- um. By studying the several factors affecting survival of shoot tips, it was found that MS medium supplemented with 0.5 mg/L BA, 0.01 mg/L NAA and 30 g/L sucrose were suit- able for the culture of shoot tips; shoot tips which were cut to 0.3 mm could used for virus- free culture. Moreover, higher survival rate of shoot tips was observed in the heat treat- ment of 38 ℃ air temperature for 38 days before the shoot tips were cut to 0.05 mm, heat pre-treatment is the efficient way for virus-free culture of strawberry ＇Darselect＇.