| 应用LAMP法鉴定牛早期胚胎性别试验 |
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| 引用本文： | 徐惊涛.应用LAMP法鉴定牛早期胚胎性别试验[J].青海畜牧兽医杂志,2011,41(5):18-19. |
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| 作者姓名： | 徐惊涛 |
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| 作者单位： | 青海省畜牧兽医科学院,西宁,810016 |
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| 基金项目： | 科技部国际科技交流与合作专项:“利用体外受精生产良种犏牛胚胎技术研究”2008DFA31100 |
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| 摘 要： | 使用LAMP法对12枚牛体外受精胚胎的基因扩增,通过使用不同的引物,对牛胚胎中的雄性特异性核酸序列和雌雄共有的核酸序列进行扩增,鉴定胚胎的性别,结果7枚为雌性,5枚雄性。
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| 关 键 词： | LAMP法 牛早期胚胎 性别鉴定 |
Experiment of Bovine Embryo Sex Determination by LAMP Method |
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| XU Jing-tao.Experiment of Bovine Embryo Sex Determination by LAMP Method[J].Chinese Qinghai Journal of Animal and Veterinary Sciences,2011,41(5):18-19. |
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| Authors: | XU Jing-tao |
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| Institution: | XU Jing-tao(Qinghai Academy of Animal Science and Veterinavy,Xining,810016) |
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| Abstract: | Gene amplification of 12 IVF frozen bovin embryoes were used to determine sex by LAMP method.Male-specific nucleic acid sequences and Female,Male Sharing nucleic acid sequences in embryo were amplified to identify embryo sex.The results indicated that there were 7 females and 5 males. |
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| Keywords: | LAMP Early bovine embryoes Sex identification |
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