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Occurrence of Cucurbit Yellow Stunting Disorder Virus (CYSDV) and Beet Pseudo-yellows Virus in Cucurbit Crops in Spain and Transmission of CYSDV by Two Biotypes of Bemisia tabaci
Authors:Benjamín Berdiales  Juan J Bernal  Elisa Sáez  Bert Woudt  Francisco Beitia  Emilio Rodríguez-Cerezo
Institution:(1) Centro Nacional de Biotecnología (CNB-CSIC), Campus de Cantoblanco, 28049 Madrid, Spain;(2) Servicio de Protección de los Vegetales, El Ejido-Almería, Spain;(3) S&G Novartis Seeds, Enkhuizen, The Netherlands;(4) CIT-INIA, Ctra Coruña km 7.5, 28040 Madrid, Spain
Abstract:The relative occurrence in Spain of two whitefly transmitted closteroviruses causing similar yellowing diseases in melon and cucumber greenhouse crops was studied. Based on a RT-PCR assay, a 1994–1997 survey of Spanish greenhouses showed that the recently described Bemisia tabaci-transmitted cucurbit yellow stunting disorder virus (CYSDV) has displaced the Trialeurodes vaporariorum-transmitted beet pseudo-yellows virus (BPYV), a virus that was present in the area since the late 1970s. The CYSDV transmission rates by each of the two biotypes of B. tabaci present in Spain were compared. The results showed that the ubiquitous lsquoBrsquo biotype and the resident lsquoQrsquo biotype (found in Spain and Portugal) were able to transmit CYSDV with similar efficiency.
Keywords:closterovirus  crinivirus  Trialeurodes  whiteflies
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