| 大孔树脂对印楝素A吸附纯化的研究 |
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| 引用本文： | 王秋芬,宋湛谦.大孔树脂对印楝素A吸附纯化的研究[J].林产化学与工业,2006,26(1):53-56. |
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| 作者姓名： | 王秋芬 宋湛谦 |
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| 作者单位： | 1. 中国林业科学研究院,林产化学工业研究所,江苏,南京,210042;济南大学化学化工学院,山东,济南,250022
2. 中国林业科学研究院,林产化学工业研究所,江苏,南京,210042 |
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| 基金项目： | 中国林科院资源昆虫培育与利用重点实验室资助项目（2002-1） |
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| 摘 要： | 通过比较5种大孔吸附树脂对印楝素A的吸附率和解吸率,成功地筛选出比较理想的树脂。研究结果表明:XAD-1180树脂对印楝素A有较好的吸附和解吸效果。并对其动态吸附、解吸性能进行了考察,发现较佳的吸附条件为:印楝素A质量浓度2.23 mg/mL(溶剂为30%甲醇-水溶液,以下同),流速1 BV/h,饱和吸附量4.5~5 BV;解吸条件为:以50%、60%、70%的甲醇-水溶液梯度洗脱。一次提纯产品的纯度为85.14%,经过二次提纯的纯度可达93.18%。纯化产物经HPLC-MS进一步确认为印楝素A。
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| 关 键 词： | 印楝素A 大孔吸附树脂 动态吸附 |
| 文章编号： | 0253-2417（2006）01-0053-04 |
| 收稿时间： | 10 19 2004 12:00AM |
| 修稿时间： | 2004-10-19 |
Study on Adsorption and Purification of Azadirachtin A by Macroporous Resin |
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| WANG Qiu-fen,SONG Zhan-qian.Study on Adsorption and Purification of Azadirachtin A by Macroporous Resin[J].Chemistry & Industry of Forest Products,2006,26(1):53-56. |
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| Authors: | WANG Qiu-fen SONG Zhan-qian |
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| Institution: | 1. Institute of Chemical Industry of Forest Products,CAF, Nanjing 210042, China; 2. College of Chemistry and Chemical Engineering, Jinan University, Jinan 250022, China |
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| Abstract: | Five types of macroporous resin were studied in order to compare their performances in absorbing and desorbing(azadirachtin) A.XAD-1180 was considered as the optimal macroporous resin for azadirachtin A.The dynamic(adsorption) and(desorption) conditions for purification of azadirachtin A were studied.The appropriate adsorption conditions were: concentration of azadirachtin A 2 mg/mL in 30% methanol solution,flow rate 1 BV/h,amount of saturated adsorption toward azadirachtin A 4-5 BV.The desorption conditions were: gradient elution with 50%,60% and 70% methanol solution at flow rates 1 and(0.5 BV/h).The purity of azadirachtin A purified by XAD-1180 for one time was 85.15%.Its purity was 93.1% when purified twice.The purified product was further confirmed to be azadirachtin A by HPLC-MS. |
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| Keywords: | azadirachtin A macroporous resin dynamic absorbing |
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