Characterization,cerebral distribution and gonadotropin release activity of neuropeptide Y (NPY) in the goldfish |
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Authors: | O Kah A Pontet J -M Danger P Dubourg G Pelletier H Vaudry A Calas |
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Institution: | (1) Laboratoire de Physiologie des Interactions Cellulaires, UA CNRS 339, Avenue des Facultés, 33405 Cédex, France;(2) Groupe de Recherche en Endocrinologie Moléculaire, UA CNRS 650, Université de Rouen, 76134 Mont Saint-Aignan, France;(3) MRC Group in Molecular Endocrinology, CHU Laval, Québec, GIV 4G2, Canada;(4) Laboratoire de Physiologie Cellulaire et Comparée, UA CNRS 651, Parc Valrose, 06034 Nice Cédex, France |
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Abstract: | The presence of a peptide closely related to porcine NPY has been demonstrated in the goldfish brain and pituitary by means
of radioimmunoassay (RIA) and high performance liquid chromatography (HPLC). The RIA data demonstrate that displacement curves
of brain extracts are parallel to a porcine NPY standard and that in HPLC a compound present in brain extracts is co-eluted
with porcine NPY. The distribution of this NPY-like factor within the central nervous system was studied by radioimmunoassay
and immunohistochemistry. The results indicated that NPY has a widespread distribution with the highest concentrations being
found in the telencephalon and diencephalon. In the pituitary gland, NPY immunoreactive terminals characterized at the electron
microscope level were found in the different lobes and, in particular, in close association with the gonadotrophin (GTH) secreting
cells. Using anin vitro perifusion system, it was shown that NPY causes a dose dependent increase of GTH release from anterior lobe fragments.
These data indicate for the first time in teleosts that NPY is present and widely distributed in the brain and pituitary,
and that among other putative functions, could be implicated in the multihormonal release of GTH from the pituitary. |
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Keywords: | neuropeptide Y goldfish gonadotrophin release neuroendocrine regulation pituitary immunocytochemistry high performance liquid chromatography radioimmunoassay |
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