Prevalence of canine coronavirus (CCoV) in dog in Japan: detection of CCoV RNA and retrospective
serological analysis |
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Authors: | Tomomi TAKANO Saya YAMASHITA Michiko MURATA-OHKUBO Kumi SATOH Tomoyoshi DOKI Tsutomu HOHDATSU |
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Institution: | 1)Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034–8628, Japan |
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Abstract: | We collected rectal swabs from dogs in Japan during 2011 to 2014, and canine coronavirus (CCoV) nucleocapsid
gene was detected by RT-PCR. The relationship between CCoV infection and the manifestation of diarrhea
symptoms was investigated, and a correlation was noted (df=1, χ2=8.90,
P<0.005). The types of CCoV detected in samples from CCoV-infected dogs were CCoV-I in
88.9% and CCoV-II in 7.4%, respectively. We retrospectively investigated the seroprevalence of CCoV-I in dogs
in Japan during 1998 to 2006. The sera were tested with a neutralizing antibody test. In the absence of CCoV-I
laboratory strain, we used feline coronavirus (FCoV)-I that shares high sequence homology in the S protein
with CCoV-I. 77.7% of the sera were positive for neutralizing anti-FCoV-I antibodies. |
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Keywords: | canine coronavirus RT-PCR serological prevalence serotype |
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