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Fine mapping of the gene for susceptibility to black spot disease in Japanese pear (Pyrus pyrifolia Nakai)
Authors:Shingo Terakami  Shigeki Moriya  Yoshihiko Adachi  Miyuki Kunihisa  Chikako Nishitani  Toshihiro Saito  Kazuyuki Abe  Toshiya Yamamoto
Institution:1.NARO Institute of Fruit Tree Science, Tsukuba, Ibaraki 305-8605, Japan;2.Apple Research Station, NARO Institute of Fruit Tree Science, Shimokuriyagawa, Morioka, Iwate 020-0123, Japan;3.Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, Shimizuokitsunaka-cho, Shizuoka, Shizuoka 424-0292, Japan
Abstract:Black spot disease, which is caused by the Japanese pear pathotype of the filamentous fungus Alternaria alternata (Fries) Keissler, is one of the most harmful diseases in Japanese pear cultivation. We mapped a gene for susceptibility to black spot disease in the Japanese pear (Pyrus pyrifolia Nakai) cultivar ‘Kinchaku’ (Aki gene) at the top of linkage group 11, similar to the positions of the susceptibility genes Ani in ‘Osa Nijisseiki’ and Ana in ‘Nansui’. Using synteny-based marker enrichment, we developed novel apple SSR markers in the target region. We constructed a fine map of linkage group 11 of ‘Kinchaku’ and localized the Aki locus within a 1.5-cM genome region between SSR markers Mdo.chr11.28 and Mdo.chr11.34. Marker Mdo.chr11.30 co-segregated with Aki in all 621 F1 plantlets of a ‘Housui’ × ‘Kinchaku’ cross. The physical size of the Aki region, which includes three markers (Mdo.chr11.28, Mdo.chr11.30, and Mdo.chr11.34), was estimated to be 250 Kb in the ‘Golden Delicious’ apple genome and 107 Kb in the ‘Dangshansuli’ Chinese pear genome. Our results will help to identify the candidate gene for susceptibility to black spot disease in Japanese pear.
Keywords:Alternaria alternata  black spot disease  Pyrus pyrifolia  fine mapping  comparative genomics
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