Study on Cell Suspension Culture of Floribunda Rose |
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Authors: | ZHANG Chun'ai WANG Jingang FAN Jinping GONG Shufang CHE Daidi |
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Affiliation: | College of Horticulture,Northeast Agricultural University,Harbin 150030,China |
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Abstract: | Friable callus was induced when immature seeds of floribunda rose were inoculated on MS medium supplemented with 2,4-D 3.0 mg-L-1.When transfered onto subculture media,fi-iable callus developed into embryogenic callus,which was used to establish cell suspension lines.Cell suspensions had to be subcultured at a interval of 4-5 days at the first several culture cycles.The best subculturing cycle for the stable cell suspensions was 8-10 days.The best inoculum quantity was 1 mL PCV(Packed Cell Volume) per 40 mL culture fluid. |
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Keywords: | floribunda rose embryogenic callus cell suspension culture |
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