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Preliminary studies on establishment of genetic transformation system in loblolly pine |
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| Authors: | Tang Wei Tian Yingchuan Ouyang Fan |
| Affiliation: | (1) State key Laboratory of Biochemical Engineering. Institute of Chemical Metallurgy, Chinese Academy of Sciences, 100080 Beijing, P.R. China;(2) Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing;(3) State key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, 100080 Beijing, P.R. China |
| Abstract: | A transformation system was established for loblolly pine (Pinus taeda L.) mature zygotic embryos usingAgrobacterium tumefaciens. The gene coding for the β-glucuronidase (GUS) gene was introduced into loblolly pine tissues and its transient expression was detected with histochemical staining. The influences of different genotypes.Agrobacterum concentrations. and cocultivation time on GUS expression and kanamycin resistant callus and shoot regeneration were investigated. The results showed that the highest GUS expression frequency (16.3%) and shoot regeneration frequency (78%) were obtained from genotype 9–1003 withAgrobacterium concentration decreased 9 times and cocultivation time of 56 hours respectively. GUS expression was obtained in all genotypes tested. The successful expression of the GUS gene in different genotypes suggested that it will be a useful transformation system for loblolly pine. (Responsible Editor: Chai Ruihai) |
| Keywords: | Pinus taeda L. Genetic transformation Agrobacterium tumefaciens |
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