| 基因表达系列分析技术及其在植物抗病性研究中的应用 |
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| 引用本文： | 李星,李亚宁,杨文香,刘大群. 基因表达系列分析技术及其在植物抗病性研究中的应用[J]. 农业生物技术学报, 2006, 14(5): 803-808 |
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| 作者姓名： | 李星 李亚宁 杨文香 刘大群 |
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| 作者单位： | 河北农业大学植物病理系,河北省农作物病虫害生物防治工程技术研究中心,保定,071001 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 基因表达系列分析(SAGE)技术不仅能够全面地分析特定组织或细胞内表达的基因，还可以比较不同组织在不同时间、空间条件下基因表达的差异，从而发现新基因。SAGE技术在植物抗病性研究中的应用近几年发展很快。综述介绍了SAGE技术的原理、特点、实验方案、技术发展与演变。
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| 关 键 词： | 基因表达系列分析(SAGE)；植物抗病性；全基因组表达谱；差异表达 |
| 文章编号： | 1006-1304（2006）05-0803-06 |
| 收稿时间： | 2005-11-25 |
| 修稿时间： | 2005-12-20 |
Serial Analysis of Gene Expression Technology and Its Application in the Research of Plant Disease Resistance |
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| LI Xing,LI Ya-ning,YANG Wen-xiang,LIU Da-qun. Serial Analysis of Gene Expression Technology and Its Application in the Research of Plant Disease Resistance[J]. Journal of Agricultural Biotechnology, 2006, 14(5): 803-808 |
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| Authors: | LI Xing LI Ya-ning YANG Wen-xiang LIU Da-qun |
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| Affiliation: | Department of Plant Pathology, Agricultural University of Hebei, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Baoding 071001, China |
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| Abstract: | Serial analysis of gene expression (SAGE) can be used not only to analyse gene expression in specific cells or tissues, but also to simultaneously compare the differential genes expressed in different tissues and different conditions for gene mining. In recent years, the application of SAGE in the research of plant disease resistance is enhancing rapidly. The review introduces principle, characteristic, protocol and methodological evolution of SAGE. And the applications of prospect of SAGE technology in the research of plant disease resistance are also discussed. |
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| Keywords: | serial analysis of gene expression(SAGE) plant disease resistance whole-profile of gene expression differential expression |
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