| 猪感染类猪圆环病毒因子P1后血小板的变化 |
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| 引用本文： | 温立斌,何孔旺,杨汉春,吕立新,郭容利,周俊明,钟书霖.猪感染类猪圆环病毒因子P1后血小板的变化[J].内蒙古农业科技,2009(3):54-56. |
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| 作者姓名： | 温立斌 何孔旺 杨汉春 吕立新 郭容利 周俊明 钟书霖 |
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| 作者单位： | 1. 江苏省农业科学院,兽医研究所,江苏,南京,210014;农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210014;国家兽用生物制品工程技术研究中心,江苏南京 210014
2. 中国农业大学,动物医学院,北京,100094;农业部预防兽医学重点开放实验室,北京,100094 |
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| 基金项目： | 国家"973"计划前期研究专项，江苏省自然科学基金 |
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| 摘 要： | 探讨类猪圆环病毒因子P1感染对广西巴马小型猪血小板参数的影响。将12头30日龄的广西巴马小型猪随机、平均分成两组．实验组经腹股沟淋巴结途径接种类猪圆环病毒因子P1的分子克隆。采用自动血液分析仪检测接种后3d、8d、17d、24d、31d及40d猪的外周血血小板参数。统计分析显示猪感染P1后，与对照组相比。实验组的血小板平均体积（MPV）无显著差异，而血小板计数（PLT）和血小板压积（PCT）在接种后8d明显降低，血小板体积分布宽度（PDW）在接种后17d显著降低。
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| 关 键 词： | 类猪圆环病毒因子 广西巴马小型猪 血小板参敷 |
Platelet Change of Porcine Circovirus Type 2-like P1 Infected Swine |
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| Institution: | WEN Li-bin (1.Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China; 2.Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agricuhure,Nanjing 210014,China; 3.National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014,China) |
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| Abstract: | This experiment was to explore platelet parameters change in Guangxi Bama miniature pig infected by porcine circovirus-like agent P1.Twelve Guangxi Bama miniature pigs at 1 month of age were randomly designated to two groups of 6 each. Group 1 pigs were each injected into the superficial inguinal lymph nodes with the cloned P1 plasmid DNA and pigs in group 2 served as controls. The peripheral blood platelet parameters of the pigs were detected with the automatic hematology analytic instrument at 3,8,17,24,31 and 40 days postinoculation （dpi）.The statistic results showed that the mean platelet volume（MPV） were no significant difference between two groups. However, the platelet total（PLT） and plateletcrit（PCT） in porcine circovirus-like agent P1 infection were significantly decreased at 8 dpi （P〈0.05） and platelet distribution width （PDW） be at 17 dpi （V〈0.05）. |
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| Keywords: | Porcine circovirus-like agent Guangxi Bama miniature pig Platelet parameters |
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