| 黄瓜雌核发育早期过氧化物同工酶活性的变化 |
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| 引用本文： | 王晓云,杜胜利,曹翠玲,魏爱民,韩毅科,张桂华. 黄瓜雌核发育早期过氧化物同工酶活性的变化[J]. 华北农学报, 2004, 19(4): 55-58 |
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| 作者姓名： | 王晓云 杜胜利 曹翠玲 魏爱民 韩毅科 张桂华 |
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| 作者单位： | 西北农林科技大学,陕西,杨凌,712100;天津科润黄瓜研究所,天津,300192 |
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| 基金项目： | 天津市农业生物技术研究中心科技(攻关)计划项目(003122011) |
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| 摘 要： | 以离体培养的黄瓜未受精子房为试材，测定了黄瓜离体雌核发育早期过氧化物酶及其同工酶的活性。结果表明，在培养的0～9d，总的过氧化物酶(POD)、NADH氧化酶(NADHPOD)和生长素氧化酶(IAAO)活性均呈上升趋势；细胞色素氧化酶(COX)、抗坏血酸氧化酶(ASP)活性均呈下降趋势；谷胱甘肽过氧化物酶(GSH-Px)则先升后降。雌核发育早期过氧化物酶及其同工酶活性，尤其是NADHPOD和GSH-Px活性的急剧升高是雌核发育的重要标志。
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| 关 键 词： | 黄瓜 雌核发育 过氧化物酶 同工酶 离体培养 |
| 文章编号： | 1000-7091(2004)04-0055-04 |
Activities of Peroxidase and Its Isoenzymes in Early Stage of Cucumber Gynogenesis |
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| WANG Xiao-yun,DU Sheng-li,CAO Cui-ling,WEI Ai-min,HAN Yi-ke,ZHANG Gui-hua. Activities of Peroxidase and Its Isoenzymes in Early Stage of Cucumber Gynogenesis[J]. Acta Agriculturae Boreali-Sinica, 2004, 19(4): 55-58 |
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| Authors: | WANG Xiao-yun DU Sheng-li CAO Cui-ling WEI Ai-min HAN Yi-ke ZHANG Gui-hua |
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| Affiliation: | WANG Xiao-yun~1,DU Sheng-li~2,CAO Cui-ling~1,WEI Ai-min~2,HAN Yi-ke~2,ZHANG Gui-hua~2 |
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| Abstract: | Peroxidase and its isoenzymes activities were measured in gynogenesis of cucumber ovary cultured for 0-9 days.The results showed that the activities of total peroxidase (POD),NADH peroxidase (NADHPOD), indole-3-acetic acid peroxidase (IAAO) were all increasing markedly.While cytochrome peroxidase (COX) and ascorbate peroxidase (ASP) always decreased.And glutathione peroxidase (GSH-Px) increased first ,then decreased. These results indicated that it was signs of cucumber gynogenesis that activities of total peroxidase and its isoenzymes, especially NADHPOD and GSH-Px increased surprisely. |
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| Keywords: | Cucumber Gynogenesis Peroxidase Isoenzymes Isolated culture |
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