Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene |
| |
Authors: | T J Velu L Beguinot W C Vass M C Willingham G T Merlino I Pastan D R Lowy |
| |
Institution: | Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, MD 20892. |
| |
Abstract: | The epidermal growth factor (EGF) receptor gene EGFR has been placed in a retrovirus vector to examine the growth properties of cells that experimentally overproduce a full-length EGF receptor. NIH 3T3 cells transfected with the viral DNA or infected with the corresponding rescued retrovirus developed a fully transformed phenotype in vitro that required both functional EGFR expression and the presence of EGF in the growth medium. Cells expressing 4 x 10(5) EGF receptors formed tumors in nude mice, while control cells did not. Therefore, the EGFR retrovirus, which had a titer on NIH 3T3 cells that was greater than 10(7) focus-forming units per milliliter, can efficiently transfer and express this gene, and increased numbers of EGF receptors can contribute to the transformed phenotype. |
| |
Keywords: | |
|
|