解淀粉芽孢杆菌HAB-7对18株植物病原真菌的抑制作用 |
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引用本文: | 刘文波,秦春秀,熊燕红,孙 亮,林春花,缪卫国,郑服丛.解淀粉芽孢杆菌HAB-7对18株植物病原真菌的抑制作用[J].热带作物学报,2017,37(2):52-57. |
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作者姓名: | 刘文波 秦春秀 熊燕红 孙 亮 林春花 缪卫国 郑服丛 |
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作者单位: | 1 海南省热带生物资源可持续利用重点实验室/海南大学环境与植物保护学院 海南海口 570228;1 海南省热带生物资源可持续利用重点实验室/海南大学环境与植物保护学院 海南海口 570228;1 海南省热带生物资源可持续利用重点实验室/海南大学环境与植物保护学院 海南海口 570228;2 中国热带农业科学院橡胶研究所 海南儋州 571737;1 海南省热带生物资源可持续利用重点实验室/海南大学环境与植物保护学院 海南海口 570228;1 海南省热带生物资源可持续利用重点实验室/海南大学环境与植物保护学院 海南海口 570228;1 海南省热带生物资源可持续利用重点实验室/海南大学环境与植物保护学院 海南海口 570228 |
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摘 要: | HAB-7是一株分离自豇豆根际土壤、具有较强抑菌作用的解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。研究HAB-7对18株植物病原真菌及其发酵液粗提蛋白对植物病原的抑制作用,并测定其对番茄种子的促生作用。采用平板对峙法测定其对18株植物病原真菌的抑制活性,以橡胶树炭疽菌为指示菌,对其发酵上清液经60%、80%硫酸铵盐析获得的抗菌粗蛋白进行测试;并将HAB-7菌液稀释成6个浓度梯度,分别用来对番茄种子进行促生测试。结果显示:HAB-7对18株植物病原真菌的平均抑菌率为61.03%;
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关 键 词: | 解淀粉芽孢杆菌 抑菌活性 抑真菌蛋白 促生作用 |
Analysis of Antifungal Activity of Bacillus amyloliquefaciens HAB-7 Against 18 Phytopathogens |
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Abstract: | HAB-7 is a strain of Bacillus amyloliquefaciens isolated from the rhizosphere of Vigna unguiculata and has a strong inhibitory effect on phytopathogen. The strain HAB-7 was tested against 18 phytopathogenic fungi, and the crude protein extracted from the fermented HAB-7 was also tested against the phytopathogens to observe their inhibitory activities. The tomato seeds were treated with the strain HAB-7 to observe their germination and growth. Dural culture assay was used to test the antibacterial activity of HAB-7 against 18 plant pathogens. The antifungal crude protein extracted from the supernatant of fermented HAB-7 via precipitation of 60% or 80% ammonium sulfate was tested against the fungi of rubber anthracnose caused by Colletotrichum gloeosporioides Penz. CGR-1 strain which was used as an indicator pathogen. The HAB-7 was diluted into 6 different concentrations, with which tomato seeds were treated to observe their germination and growth. The HAB-7 had an average inhibition rate of 61.03% against the 18 fungi; the supernatant of the fermented HAB-7 inhibited 56.80% of C. gloeosporioides; the crude protein of HAB-7 extracted via 80% ammonium sulfate precipitation also inhibited 38.88% of C. gloeosporioides. HAB-7 bacterial suspension at 1×104 CFU/mL improved the radical growth of the tomato seeds, 67.76% longer than the control. The HAB-7 displayed a broad-spectrum antagonistic activity, and its secreted antagonistic active substance contained antifungal protein and probably other active substances since HAB-7 bacterial liquid at a low concentration promoted plant root growth. This study plays an important role in follow-up study about its pathogenicity. |
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Keywords: | Bacillus amyloliquefaciens antifungal activity antifungal protein growth promotion |
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