| 利用药物印迹法筛选化学杂交剂苯哒嗪靶标蛋白基因 |
| |
| 引用本文： | 赵新亮,张改生,宋瑜龙,李志宽,朱启迪,赵卓军,马守才,王军卫,牛娜. 利用药物印迹法筛选化学杂交剂苯哒嗪靶标蛋白基因[J]. 农药学学报, 2014, 16(6): 713-719. DOI: 10.3969/j.issn.1008-7303.2014.06.13 |
| |
| 作者姓名： | 赵新亮 张改生 宋瑜龙 李志宽 朱启迪 赵卓军 马守才 王军卫 牛娜 |
| |
| 作者单位： | 1.西北农林科技大学/国家杨凌农业生物技术育种中心/国家小麦改良中心杨凌分中心/小麦育种教育部工程研究中心/陕西省作物杂种优势研究与利用重点实验室, 陕西 杨凌 712100 |
| |
| 基金项目： | 国家高技术研究发展计划(“863”计划)重大专项(2011AA10A106);国家自然科学基金项目(31171611, 31371697);陕西省 “13115”科技创新工程与科技统筹重大科技专项(2010ZDKG-68,2014KTZB02-01-02). |
| |
| 摘 要： | 为了筛选化学杂交剂苯哒嗪的靶标蛋白基因,以SQ-1(15%苯哒嗪乳油)处理的小麦品种西农1376为材料,以噬菌体λTripl Ex2为载体,利用SMART技术构建小麦单核期花药c DNA表达文库;以生物素为报告基团,合成苯哒嗪小分子探针,并利用该探针在c DNA表达文库中进行苯哒嗪靶标蛋白基因筛选。结果成功构建了一种新型有效的药物印迹筛选方法,获得了一条能够与苯哒嗪结合的蛋白基因c DNA序列,其编码蛋白属于DUF3456家族。研究结果为进一步明确SQ-1(有效成分苯哒嗪)的作用机理提供了依据。
|
| 关 键 词： | 小麦 化学杂交剂 SQ-1 苯哒嗪 药物印迹 cDNA表达文库 靶标蛋白基因 |
| 收稿时间： | 2014-07-31 |
| 修稿时间： | 2014-10-10 |
Screening target-protein gene of chemical hybridizing agent clofencet by drug-western method |
| |
| ZHAO Xinliang,ZHANG Gaisheng,SONG Yulong,LI Zhikuan,ZHU Qidi,ZHAO Zhuojun,MA Shoucai,WANG Junwei and NIU Na. Screening target-protein gene of chemical hybridizing agent clofencet by drug-western method[J]. Chinese Journal of Pesticide Science, 2014, 16(6): 713-719. DOI: 10.3969/j.issn.1008-7303.2014.06.13 |
| |
| Authors: | ZHAO Xinliang ZHANG Gaisheng SONG Yulong LI Zhikuan ZHU Qidi ZHAO Zhuojun MA Shoucai WANG Junwei NIU Na |
| |
| Affiliation: | 1.Northwest Agriculture and Forestry University/National Breeding Center of Agricultural Biotechnology in Yangling/Yangling Branch of State Wheat Improvement Center/Wheat Breeding Engineering Research Center, Ministry of Education/Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling 712100, Shaanxi Province, China2.Department of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, Henan Province, China |
| |
| Abstract: | In order to select the target gene of chemical hybridizing agent clofencet, the cDNA expression library of uninucleate-stage anther from wheat varieties Xinong 1376 treated with SQ-1 (15% clofencet EC) was constructed by SMART technology using the carrier phage λ TriplEx2. The clofencet target genes were screened by a synthetic small molecule probes with a reporter group biotin. A novel method for effective drug screening blots was set up, and a target gene of clofencet was obtained, which encoded a protein belongs to the DUF3456 family. The results provided basis for further study on the mechanisms of SQ-1 (the active ingredient of clofencet). |
| |
| Keywords: | wheat chemical hybridizing agent SQ-1 clofencet drug-western method cDNA expression library target-protein gene |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《农药学学报》浏览原始摘要信息 |
|
点击此处可从《农药学学报》下载全文 |
|
