Hormone-sensitive lipase is involved in the action of hydroxysafflor yellow A (HYSA) inhibiting adipogenesis of 3T3-L1cells |
| |
| Institution: | 1. Department of Endocrinology, Key Laboratory of Endocrinology of Ministry of Health, The Translational Medicine Center of PUMCH, Peking Union Medical College Hospital, Beijing 100730, China;2. Chinese Academy of Medical Sciences, Beijing 100730, China;3. Peking Union Medical College, Beijing 100730, China;4. Department of Pharmacology, Beijing Anzhen Hospital, Capital Medical University, China-Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing 100029, China;1. Department of Tumor Pathology, Hamamatsu University School of Medicine, Hamamatsu, Japan;2. Research Equipment Center, Hamamatsu University School of Medicine, Hamamatsu, Japan;3. Division of Carcinogenesis and Prevention, National Cancer Center Research Institute, Tokyo, Japan;1. Department of Medicine, UT Health at San Antonio, TX, United States;2. VA Biomedical Laboratory Research, South Texas Veterans Health Care System, San Antonio, TX, United States;3. Geriatric Research, Education and Clinical Research, South Texas Veterans Health Care System, San Antonio, TX, United States;4. Department of Pathology, UT Health at San Antonio, TX, United States;1. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba City, Ibaraki, 305-8572, Japan;2. Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba City, Ibaraki, 305-8572, Japan;3. Alliance for Research on North Africa (ARENA), University of Tsukuba, Tsukuba City, Ibaraki, 305-8572, Japan |
| |
| Abstract: | BackgroundSafflor yellow A (SY) has been demonstrated to be beneficial to cardiovascular system. Our previous study showed that hydroxysafflor yellow A (HSYA), a main component of SY, could increase peroxisome proliferator-activated receptor γ mRNA expression. In this study, we investigate the effect of HSYA on the proliferation and adipogenesis of mouse 3T3-L1 preadipocytes.MethodsThe proliferation and adipogenesis of 3T3-L1 cells treated with HSYA was studied by 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide (MTT) spectrophotometry, Oil Red O staining and intracellular triglyceride assay methods. HSL mRNA expression and promoter activity were studied by real-time quantitative RT-PCR, transient transfection and dual luciferase reporter gene methods.ResultsHSYA (0.1 mg/L) significantly inhibited the proliferation of 3T3-L1 cells when compared with control cells in 8 h. This effect was further enhanced with the extension time (24 to 96 h) and an increase of concentration of HSYA (1–10 mg/L). The maximal inhibitory action was observed at 0.1 mg/L HSYA in 72 h (86 ± 11.8% vs. 100 ± 4.1%, p < 0.01). HSYA notably reduced the amount of intracellular lipid and triglyceride content in adipocytes to 85% (1 mg/L) and 75% (100 mg/L) on Day 4 following the differentiation, respectively, while increased HSL mRNA expression and promoter activities to 2.7 fold and 1.55 fold, respectively (p < 0.01), in differentiated 3T3-L1 adipocytes.ConclusionsHSYA inhibits the proliferation and adipogenesis of 3T3-L1 preadipocytes. The inhibitory action of HYSA on adipogenesis may be due to the promotion of lipolytic-specific enzyme HSL expression by increasing HSL promoter activity. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
