| ‘甜查理’草莓茎尖培养与快速繁殖研究 |
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| 引用本文： | 晁慧娟,刘敏,姬谦龙,高遐虹,董清华. ‘甜查理’草莓茎尖培养与快速繁殖研究[J]. 北京农学院学报, 2008, 23(2) |
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| 作者姓名： | 晁慧娟 刘敏 姬谦龙 高遐虹 董清华 |
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| 作者单位： | 1. 北京农学院植物科学技术系,北京,102206
2. 北京农学院组培实验中心,北京,102206 |
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| 基金项目： | 北京市教委科技发展计划项目 , 北京农学院研究生教育创新平台项目 |
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| 摘 要： | 对‘甜查理’草莓茎尖进行组织培养研究,建立其再生快繁体系。结果表明:(1)剥取的茎尖越大,萌发率越高;(2)暗培养3 d能够抑制褐化,提高萌发率;(3)增殖培养基:MS+6-BA 1.0 mg/L+NAA0.10 mg/L,增殖系数为6.13;(4)生根培养基:1/2 MS,生根率为100%,移栽到消毒过的蛭石基质,成活率为95%。
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| 关 键 词： | ‘甜查理’ 草莓 茎尖培养 快速繁殖 |
Stem Tip Culture and Rapid Propagation of Strawberry 'Sweet charlie' |
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| CHAO Hui-juan,LIU Min,JI Qian-long,GAO Xia-hong,DONG Qing-hua. Stem Tip Culture and Rapid Propagation of Strawberry 'Sweet charlie'[J]. Journal of Beijing Agricultural College, 2008, 23(2) |
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| Authors: | CHAO Hui-juan LIU Min JI Qian-long GAO Xia-hong DONG Qing-hua |
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| Abstract: | The system of rapid propagation had been established by stem tip of strawberry 'Sweet charlie'.The results showed that the best media or treatment for various stages were as follows:(1)The larger the stem tips,the higher the survival rate;(2)Darkness treatment for 3 d could inhibit browning rate and increase survival rate;(3)Proliferation medium: MS 6-BA 1.0 mg/L NAA 0.10 mg/L,in which the proliferation coefficient was 6.13;(4)Rooting medium: 1/2 MS,in which the rooting rate reached 100% and transplanted plants with root to substrate of vermiculite disinfected,the surviving rate was up to 95%. |
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| Keywords: | sweet charlie strawberry stem tip culture rapid propagation |
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