| 洋水仙Arkle离体快速繁殖体系的建立 |
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| 引用本文： | 孙晓梅,孙 琪,杨宏光.洋水仙Arkle离体快速繁殖体系的建立[J].西北农林科技大学学报(社会科学版),2010,38(3):173-178. |
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| 作者姓名： | 孙晓梅 孙 琪 杨宏光 |
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| 作者单位： | 沈阳农业大学林学院; |
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| 基金项目： | 沈阳市科技攻关项目(1071146-3-00) |
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| 摘 要： | 【目的】初步构建洋水仙的组培快繁体系,为洋水仙种球工厂化生产奠定基础。【方法】以洋水仙品种Arkle为试验材料,选用其叶片、花茎及鳞茎的不同部位作为外殖体,以MS、1/2MS和1/4MS为基本培养基,分别添加不同质量浓度的a-萘乙酸(NAA)、吲哚丁酸(IBA)、2,4-二氯苯氧乙酸(2,4-D)、6-苄基腺嘌呤(6-BA)和活性炭(AC),研究不同外植体以及不同培养基对小鳞茎诱导、增殖及生根的影响。【结果】以带鳞茎盘的双鳞片作外植体效果较好,鳞片部位以鳞茎外层鳞片的培养效果较好;较适宜的初代培养基为MS+6-BA3.0mg/L+NAA0.5mg/L+IBA0.2mg/L;较适宜的增殖培养基为MS+6-BA1.5mg/L+NAA0.3mg/L,其诱导率可达668.00%;适宜小鳞茎的生根培养基为1/2MS+NAA0.1mg/L+1g/LAC,其生根率可达到80%。【结论】鳞片分化小鳞茎存在着部位效应;6-BA和NAA组合对小鳞茎的诱导和增殖有良好效果;1/2MS与一定浓度的NAA和AC组合有利于生根。
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| 关 键 词： | 洋水仙 初代培养 增殖培养 生根培养 |
| 收稿时间： | 2009/8/24 0:00:00 |
Construction of rapid micropropagation system via in vitro culture for Narcissus cv.Arkle |
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| SUN Xiao-mei SUN Qi,YANG Hong-guang,CUI Wen-shan,WANG Ya-bin.Construction of rapid micropropagation system via in vitro culture for Narcissus cv.Arkle[J].Journal of Northwest Sci-Tech Univ of Agr and,2010,38(3):173-178. |
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| Authors: | SUN Xiao-mei SUN Qi YANG Hong-guang CUI Wen-shan WANG Ya-bin |
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| Institution: | SUN Xiao-mei SUN Qi,YANG Hong-guang,CUI Wen-shan,WANG Ya-bin(Forestry College,Shenyang Agricultural University,Shenyang,Liaoning 110866,China) |
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| Abstract: | Objectives] The aim of this study was to establish a Narcissus rapid tissue culture system initially and lay a certain foundation for factory production of Narcissus bulbs propagation.Method] We studied the effects of different explants and different media on bulblets induction,multiplication and rooting with Narcissus cv.Arkle as experimental material,its leaves,scapes and different parts of bulbs as explants,MS,1/2 MS and 1/4 MS,with different mass concentrations of the NAA,IBA,2,4-D,6-BA and AC as the ... |
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| Keywords: | Narcissus primary culture multiplication culture rooting culture |
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