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Analysis of genetic relationship in 12 species of Section Strobus with ISSR markers
引用本文:LIU Gui-feng DONG Jing-xiang JIANG Ying LU Yan-fang JIANG Jing ZHAO Guang-yi. Analysis of genetic relationship in 12 species of Section Strobus with ISSR markers[J]. 林业研究, 2005, 16(3): 213-215. DOI: 10.1007/BF02856817
作者姓名:LIU Gui-feng DONG Jing-xiang JIANG Ying LU Yan-fang JIANG Jing ZHAO Guang-yi
作者单位:[1]Forestry College, Northeast Forestry. University, Harbin 150040, P. R. China
基金项目:The study was supported by Introducing 0verseas Advanced Agriculture Science and Technology Program of China (948 Program) (2001-31)
摘    要:Genetic relationship of 12 species of Section Strobus was analyzed with ISSR markers. 117 loci were detected with 12 ISSR primers. Percentage of polymorphic bands (PPB) varied from 5.93% to 19.92%. P. pumila had the highest levels of genetic differentiation and P. flexilis had lowest. Total genetic diversity (Hr) of 12 species in Section Strobus was 26.21%, of which intraspecific genetic diversity (Hs) was 7.66%, and interspecific genetic diversity (DST) was 18.55%, and the genetic variation in interspecies accounted for 70.78% of the total genetic diversity. According to the cluster results of genetic distance, the 12 species were classified into two groups. The first group included P. griffithii, P.armandi, P. fenzeliana, P. kwangtungensis, P. strobus, P. monticola and P. wangii. The second group included P. albicaulis, P pumila, P. flexilis, P. sibirica and P koraiensis.

关 键 词:ISSR PPB 遗传多样性 遗传关系 DST 生物多样性 物种保护
文章编号:1007-662X(2005)03-0213-03
收稿时间:2005-01-20
修稿时间:2005-03-18

Analysis of genetic relationship in 12 species ofSection Strobus with ISSR markers
Liu Gui-feng,Dong Jing-xiang,Jiang Ying,Lu Yan-fang,Jiang Jing,Zhao Guang-yi. Analysis of genetic relationship in 12 species ofSection Strobus with ISSR markers[J]. Journal of Forestry Research, 2005, 16(3): 213-215. DOI: 10.1007/BF02856817
Authors:Liu Gui-feng  Dong Jing-xiang  Jiang Ying  Lu Yan-fang  Jiang Jing  Zhao Guang-yi
Affiliation:(1) Forestry College, Northeast Forestry University, 150040 Harbin, P.R. China
Abstract:Genetic relationship of 12 species ofSection Strobus was analyzed with ISSR markers. 117 loci were detected with 12 ISSR primers. Percentage of polymorphic bands (PPB) varied from 5.93% to 19.92%.P. pumila had the highest levels of genetic differentiation andP. flexilis had lowest. Total genetic diversity (HT) of 12 species inSection Strobus was 26.21%, of which intraspecific genetic diversity (Hs) was 7.66%, and interspecific genetic diversity (DST) was 18.55%, and the genetic variation in interspecies accounted for 70.78% of the total genetic diversity. According to the cluster results of genetic distance, the 12 species were classified into two groups. The first group includedP. griffithii, P. armandi, P. fenzeliana, P. kwangtungensis, P. strobus, P. monticola andP. wangii. The second group includedP. albicaulis, P. pumila, P. flexilis, P. sibirica andP. koraiensis. Foundation item: The study was supported by Introducing Overseas Advanced Agriculture Science and Technology Program of China (948 Program) (2001-31) Biography: LIU Gui-feng (1960-), male, professor of Northeast Forestry University, Harbin 150040, P.R. China. Responsible editor: Song Funan
Keywords:Pinus   ISSR-PCR   Genetic relationship
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