| 韩国白腐菌(Trametes versicolor)的Mn-过氧化物酶同工酶特性研究 |
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| 引用本文： | JunSangcheol KimKyunghoon ChoiHyoungT. KimKyujoong JunSangcheol KimKyunghoon ChoiHyoungT. KimKyujoong. 韩国白腐菌(Trametes versicolor)的Mn-过氧化物酶同工酶特性研究[J]. 华中农业大学学报, 2004, 23(1): 146-149 |
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| 作者姓名： | JunSangcheol KimKyunghoon ChoiHyoungT. KimKyujoong JunSangcheol KimKyunghoon ChoiHyoungT. KimKyujoong |
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| 作者单位： | [1]DeptofBiology,KangnungNationalUniversity,Kangnung,210-702,Korea [2]DivofLifeScience,KangwonNationalUniversity,Chuncheon,200-701,Korea |
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| 基金项目： | Supported in part by grants from the Korea Science and Engineering Foundation (KOSEF 1999-2-209-002-3)，from the Research Foun-dation of Kangnung National University (Yungu-81510-421). |
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| 摘 要： | 白腐担子菌Trametes versicolor在加人0．05%Tween80，(NH4)2-tatrate和80mg／kgMnSO4的Kirks培养基上能够高活性地分泌Mn-过氧化物酶。在该培养基中培养10d，活性达到最高。通过DEAE-SepharoseCL-6B，SP-Sepharose和离子交换柱分离纯化，获得2种类型的Mn-过氧化物酶同工酶，并对这2种酶的酶动力学，N-端的氨基酸序列及其编码基因的核酸序列做了研究。
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| 关 键 词： | 白腐菌 Mn-过氧化物酶 同工酶 |
Characterization of Mn-peroxidase Isoenzymes from the White-rot Fungus Trametes versicolor in Korea |
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| Abstract. Characterization of Mn-peroxidase Isoenzymes from the White-rot Fungus Trametes versicolor in Korea[J]. Journal of Huazhong Agricultural University, 2004, 23(1): 146-149 |
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| Authors: | Abstract |
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| Abstract: | The white-rot basidiomycete Trametes versicolor produced high levels of Mn-peroxidase activity when grown on Kirks media with 0. 05% Tween 80, 2. 4 mmol/L (NH4)2-tatrate, 80 mg/kg of MnSO4. Maximum activity of Mn-peroxidase was appeared on the 10th day of culture on above medium of combinations. Two types of isoenzymes of Mn-peroxidase were separated by DEAE-Sepharose CL-6B, SP-Sepharose, anion and cation exchange chromatography on Mono-Q and Mono-S column. Those iso-enzymes were characterized in view point of enzyme kinetics, N-terminal amino acid sequences, and base sequences of the coded genes, etc. |
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| Keywords: | Trametes versicolor Mn-peroxidase isoenzyme |
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