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Chlorpyrifos Detection by Piezoelectric Biosensor Based on Acetylcholinesterase Immobilization
Authors:GU Yunxiuzi  XU Yanli  SHANG Zhanjiang
Affiliation:1. College of Agriculture,Northeast Agricultural University,Harbin 150030,China;Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,China
2. Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,China
Abstract:Acetylcholinesterase (AChE) was immobilized on multilayer films assembled by poly diallyldimethylammonium chloride (PDDA) and ι-carrageenan (IC) on silver-coated crystal electrode surfaces to detect the chlorpyrifos belonging to the organophosphates pesticide.Mass sensitive quartz crystal microbalance (QCM) was used to study the effect of AChE concentration and pH of phosphate buffer solution on immobilized acetylcholinesterase.The optimized conditions were as follows: pH was 6.0 which was near isoelectric ...
Keywords:acetylcholinesterase  quartz crystal microbalance (QCM)  self-assemble  chlorpyrifos  
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