| 杨树湿地松组织培养愈伤组织耐盐性* |
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| 引用本文： | 张立钦,郑勇平,罗士元,胡加共.杨树湿地松组织培养愈伤组织耐盐性*[J].浙江农林大学学报,1997,14(1):16-21. |
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| 作者姓名： | 张立钦 郑勇平 罗士元 胡加共 |
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| 作者单位： | 1.浙江林学院林学系, 临安 311300;2.浙江省林业厅种苗站;3.浙江省台州地区林业特产局 |
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| 基金项目： | * 浙江省自然科学基金资助项目 |
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| 摘 要： | 对杨树无性系N L-80105, N L-80106, N L-80117和湿地松组织培养愈伤组织的耐盐性进行了研究。结果表明:以MS为基本培养基，添加2,4-D 2.0 mg kg-1和KT 0.5 mg kg-1，诱导这些树种愈伤组织效果好；在含氯化钠的培养基上，杨树不同器官外植体的愈伤组织诱导能力和耐盐程度有差异，外植体茎、叶比根强；杨树继代愈伤组织的耐盐能力显著高于湿地松，这与植株水平的耐盐性相一致，表明用愈伤组织鉴别这些植物耐盐性的方法是可行的。
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| 关 键 词： | 杨属 无性系 湿地松 组织培养 愈伤组织 耐盐性 |
| 收稿时间： | 1996-01-25 |
Salt Tolerance of Calli of Populus Clones and Pinus elliottii in Tissue Cultures |
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| Zhang Liqin,Zheng Yong ping,Luo Shiyuan,Hu Jiagong..Salt Tolerance of Calli of Populus Clones and Pinus elliottii in Tissue Cultures[J].Journal of Zhejiang A&F University,1997,14(1):16-21. |
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| Authors: | Zhang Liqin Zheng Yong ping Luo Shiyuan Hu Jiagong |
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| Institution: | 1.Zhejiang Forestry College, Lin ’an 311300, PRC |
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| Abstract: | The tolerance to NaCl of tissues culture calli of Populus clones (NL-80105, NL-80106, and NL-80117) and Pinus elliottii was studied. The results were as follow s: (1) MS medium with 2.0 mg kg- 1 of 2, 4-D and 0.5 mg kg- 1 KT was suitable for tree calli in tissuecul tures; (2) the salt tolerances of calli from roots, stems and petioles were different on MS containing NaCl. The calli with the highest salt to lerance was from stems; (3) the salt tolerance of subculture calli of Populus clones was higher than that of Pinus elliottii, which was in keeping with the salt tolerance of Populus and Pinus elliottii trees. |
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