|
|||||
|
|
| 甘蔗花叶病毒复制酶基因的克隆及序列分析 | |
| 引用本文： | 姚伟,段真珍,周会,何正权,张木清,陈如凯.甘蔗花叶病毒复制酶基因的克隆及序列分析[J].西北农业学报,2006,15(5):80-84. |
| 作者姓名： | 姚伟 段真珍 周会 何正权 张木清 陈如凯 |
| 作者单位： | 1. 三峡大学生物技术研究中心,宜昌,443002;福建农林大学,农业部甘蔗生理生态与遗传改良重点开放实验室,福州,350002 2. 三峡大学生物技术研究中心,宜昌,443002 3. 福建农林大学,农业部甘蔗生理生态与遗传改良重点开放实验室,福州,350002 |
| 基金项目： | 国家高技术研究发展计划(863计划);湖北省教育厅青年基金 |
| 摘 要： | 以甘蔗花叶病毒(SCMV)福建分离物(FJ)的总RNA为模板,用RT-PCR方法扩增了含有复制酶(NIb)基因的DNA片段,将其克隆到质粒pGM-T载体上并进行了序列分析。结果表明,NIb基因由1563个碱基组成,编码521个氨基酸并含有高度保守序列GDD。NIa/NIb和NIb/CP交界处的蛋白酶切割位点分别为Q/C和Q/A。与国外报道的SCMV亚组几个株系分离物相比,NIb基因(福建分离物)的核苷酸和氨基酸序列同源性分别为57.8%~86.9%和60.9%~95.2%,其中与SCMV-SA株系的核苷酸及氨基酸序列同源性则高达86.9%和95.2%。 |
| 关 键 词： | 甘蔗 甘蔗花叶病毒 复制酶因 序列测定 |
| 文章编号： | 1004-1389（2006）05-0080-05 |
| 收稿时间： | 2006-01-23 |
| 修稿时间： | 2006-04-09 |
Cloning and Sequence Analysis of Replicase Genes of Sugarcane Mosaic Virus |
|
| YAO Wei,DUAN Zhen-zhen,ZHOU Hui,HE Zheng-quan,ZHANG Mu-qing and CHEN Ru-kai.Cloning and Sequence Analysis of Replicase Genes of Sugarcane Mosaic Virus[J].Acta Agriculturae Boreali-occidentalis Sinica,2006,15(5):80-84. | |
| Authors: | YAO Wei DUAN Zhen-zhen ZHOU Hui HE Zheng-quan ZHANG Mu-qing and CHEN Ru-kai |
| Institution: | China Three Gorges University Biotechnology Research Centre, Yichang 443002, China;Key Lab of Eco-physiology & Genetic Improvement for Sugarcane, Ministry of Agriculture, P. R. China, Fujian Agriculture and Forestry University, Fuzhou 350002, China;China Three Gorges University Biotechnology Research Centre, Yichang 443002, China;Key Lab of Eco-physiology & Genetic Improvement for Sugarcane, Ministry of Agriculture, P. R. China, Fujian Agriculture and Forestry University, Fuzhou 350002, China;China Three Gorges University Biotechnology Research Centre, Yichang 443002, China;Key Lab of Eco-physiology & Genetic Improvement for Sugarcane, Ministry of Agriculture, P. R. China, Fujian Agriculture and Forestry University, Fuzhou 350002, China;Key Lab of Eco-physiology & Genetic Improvement for Sugarcane, Ministry of Agriculture, P. R. China, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
| Abstract: | The NIb coding region of sugarcane mosaic virus(SCMV) from Fujian was am plified from the extracted plant total RNA by using RT-PCR,and cloned into the pGM-T vector and sequenced.The result indicated that the NIb coding region comprised 1563 nucleotides and encoded a putative protein of 521 amino acids containing the consensus motif GDD.The protease cleavage sites between the NIa/NIb and NIb/coat protein were found to be Q/C and Q/A respectively.The nucleotide and the putative amino acid of replicase were compared with the other isolates or stain of SCMV subgroup,it shares 57.8%~86.9% and 60.9% ~95.2% homology in nucleo tide and the putative amino acid sequence respectively.The NIb sequence was most similar to SCMV-SA with identities of 86.9% and 95.2% at the nucleot ide and amino acid levels respectively. |
| Keywords: | Sugarcane Mosaic Virus Coat Protein Molecular Detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《西北农业学报》浏览原始摘要信息 | |
| 点击此处可从《西北农业学报》下载免费的PDF全文 | |