甘蔗黑穗病菌拮抗细菌的筛选及鉴定

从甘蔗根际土壤及甘蔗不同组织内分离到的928个细菌菌株中，对甘蔗黑穗病菌有拮抗作用的细菌菌株有301个．占32．4％．其中拮抗能力强(拮抗带大于10mm)的菌株有18个，占1．9％。经在KBA培养基上培养．发现具有拮抗作用的细菌主要是荧光菌和非荧光菌中的白色菌群。在18个拮抗性强的菌株中，13个菌株来自甘蔗的茎、芽(生长点)，占72％；5个菌株来自根际土壤，占28％；12个菌株为革兰氏阳性细菌中的芽孢杆菌属；6个菌株为革兰氏阴性细菌，其中4个菌株分别为假单胞杆菌属、不动杆菌属、伯克氏菌属及沙雷氏菌属，其余2个菌株有待进一步鉴定。

Screening and identification of antagonistic bacteria of Ustilago scitaminea

928 strains of bacteria were isolated from soil around root and different tissues of sugarcane.The results of antagonistic effect to Ustilago scitaminea on media plate showed that 301(32.4%) strains of bacteria were antagonistic,and 18(1.9%) strains of bacteria were strong antagonistic (the antagonistic zone is more than 10 mm).The most of antagonistic bacteria were fluorescent and the white colony group of non-fluorescent bacteria on KBA palte. Among the 18 strains of strong antagonistic bacteria, 13 (72%) strains were from bud and stem of sugarcane, 5(28%) strains were from soil around root of sugarcane; 12 strains were Gram-positive which all belong to Bacillus and 6 strains were Gram-negative in which 4 strains were Pseudomonas,Acinetobacter,Burkholderia and Serratia,respectively,the other two strains need to be identified in future.