Putative Phosphatase UvPsr1 Is Required for Mycelial Growth,Conidiation, Stress Response and Pathogenicity in Ustilaginonidea virens |
| |
Authors: | Meng Xiong Shuai Meng Jiehua Qiu Huanbin Shi Xiangling Shen Yanjun Kou |
| |
Institution: | 1.Key Laboratory of Three Gorges Regional Plant Genetics & Germplasm Enhancement / Biotechnology Research Center, China Three Gorges University, Yichang 443000, China;2.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China |
| |
Abstract: | Ustilaginoidea virens is the causal agent of rice false smut, which can be a highly destructive disease of rice. The plasma membrane phosphatase Psr1 proteins, which act as a regulator of the salinity stress response in yeast, are widely distributed across fungi, but their functional characterization is sketchy. In this study, we characterized the functions of Psr1 protein, UvPsr1, in U. virens. Analyses of the ΔUvpsr1 and its complementation strain showed that UvPsr1 is required for normal mycelial growth, conidiation and tolerance to oxidative, osmotic and cell wall stresses. When rice panicles were inoculated with the ΔUvpsr1 strains, no symptoms of false smut disease developed, showing that UvPSR1 also contributes to the pathogenicity of the fungus. The deletion mutant of UvPSR1 also appeared to produce a smaller titer of toxic compounds able to inhibit elongation of the germinated seeds. In conclusion, our results indicated that UvPsr1 is a new pathogenic factor of U. virens. |
| |
Keywords: | Psr1 rice false smut disease pathogenic factor clavicipitaceae |
本文献已被 ScienceDirect 等数据库收录! |
| 点击此处可从《水稻科学》浏览原始摘要信息 |
| 点击此处可从《水稻科学》下载免费的PDF全文 |
|