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A New Embryo Rescue Procedure for Interspecific Hybridization
Authors:Regine  Mathias  Sonsoles  Espinosa G Röbbelen
Institution:Dr. Regine;Mathias, Dipl. Ing. agr. Sonsoles Espinosa, Prof. Dr. G. Röbbelen, Institut fur Pflanzenbau und Pflanzenzüchtung, Von-Siebold-Str. 8, D-3400 Göttingen (Germany, F.R.)
Abstract:A new technique is presented that allows to overcome very early embryo abortion after interspecific crosses. Embryogenic sectors of young fertilized ovules are cultivated in vitro on a solid/liquid double layer culture medium containing activated charcoal in the solid phase. Hybrid plans are obtained afer transfer the developed for the interspecific cross Cuphea paucipetala greenwooodii×C. laminuligera; but it is most likely also applicable to other combinations, even in different genera.
Keywords:Cuphea spec  embryo rescue  interspecific hybridization              in vitro culture
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