| AtFT基因植物表达载体的构建研究 |
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| 引用本文： | 李玉婷 华玉伟 黄天带 蔡海滨 黄华孙. AtFT基因植物表达载体的构建研究[J]. 中国农学通报, 2013, 29(3): 173-179. DOI: 10.11924/j.issn.1000-6850.2012-2278 |
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| 作者姓名： | 李玉婷 华玉伟 黄天带 蔡海滨 黄华孙 |
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| 作者单位： | 中国热带农业科学院橡胶研究所,海南儋州571737 |
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| 基金项目： | 国家天然橡胶产业技术体系(nycytx-34);国家自然科学基金“橡胶树抗白粉病新种质培育”(30960322);中央级科研院组基本业务费“橡胶树高效遗传转化体系的建立”(1630022012006) |
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| 摘 要： | FT是从拟南芥克隆得到的诱导植物开花调控基因。利用FT基因表达特点，克隆拟南芥FT基因片段，重组构建了以CaMV35S为启动子、GUS为报告基因的植物表达载体pCAMBIA2301-FT与pCAMBIA2300-FT:GUS，并采用农杆菌介导法转化橡胶树体细胞胚，通过GUS组织化学染色观察橡胶树体细胞胚瞬时表达情况，结果验证了重组构建的2个载体的有效性，为进一步研究FT基因在橡胶树中的功能及应用奠定基础。
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| 关 键 词： | 孙坊镇 孙坊镇 |
| 收稿时间： | 2012-06-25 |
| 修稿时间： | 2012-07-22 |
Construction of Plant Expression Vector of AtFT Gene |
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| Li Yuting,Hua Yuwei,Huang Tiandai,Cai Haibin,Huang Huasun. Construction of Plant Expression Vector of AtFT Gene[J]. Chinese Agricultural Science Bulletin, 2013, 29(3): 173-179. DOI: 10.11924/j.issn.1000-6850.2012-2278 |
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| Authors: | Li Yuting Hua Yuwei Huang Tiandai Cai Haibin Huang Huasun |
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| Affiliation: | (Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou Hainan 571737) |
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| Abstract: | FT was an important gene involved in controlling plant flowering time from arabidopsis. This research used FT gene expression characteristics, through cloning FT gene from arabidopsis thaliana, plant expression vector pCAMBIA2301-FT and pCAMBIA2300-FT:GUS which had a CaMV 35S promoter and GUS reporter gene, were successfully constructed. The somatic embryos in Hevea were transferred by agrobacterium-mediated transformation, and the instantaneous expression of somatic embryos was observed using GUS histochemical staining. The result showed the two plants expression vectors were effective. It laid the foundation for further studying the function and application of FT genes in Hevea. |
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| Keywords: | Hevea brasiliensia FT gene plant expression vector somatic embryo |
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