Detection and identification of the phytoplasma associated with pear decline in Taiwan |
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Authors: | Hsiu-Lin Liu Ching-Chung Chen Chan-Pin Lin |
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Institution: | (1) Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, 106, Taiwan, ROC;(2) Department of Plant Protection, Taichung District Agricultural Improvement Station, Changhua, 510, Taiwan, ROC |
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Abstract: | Pear decline (PD) is an important phytoplasmal disease that occurs mainly in Europe and North America. In 1994, pear trees
exhibiting symptoms typical of PD disease were observed in orchards of central Taiwan. The sequence of 16S rDNA and 16S–23S
rDNA intergenic spacer region (ISR) of the causative agent of pear decline in Taiwan (PDTW) were amplified with polymerase
chain reaction (PCR) using a DNA template prepared from the diseased leaves. Sequence analysis of 16S rDNA revealed that the
PDTW agent was closely related to the phytoplasmas of the apple proliferation group that cause diseases in stone fruits, pear
and apple. Consistent with the result of 16S rDNA sequence analysis, sequence analysis of the 16S–23S rDNA ISR and putative
restriction site analyses of 16S rDNA and 16S–23S rDNA ISR sequences provided further support for the view that the PDTW phytoplasma
causing pear decline in Taiwan may represent a new subgroup of the apple proliferation group. According to the rDNA sequence
of PDTW phytoplasma, two specific PCR primer pairs, APf2/L1n and fPD1/rPDS1, were designed in this study for the detection
of the etiological agent in pear trees and insect vectors. Based on the sequence analyses of the PCR-amplified fragments,
two species of pear psyllas, Cacopsylla qianli and Cacopsylla chinensis, were found to carry PDTW phytoplasma. |
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Keywords: | Phytoplasma vectors rRNA |
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