Detecting RAPD Markers Linked to Ripe Rot Resistance Genes in Chinese Wild Vitis |
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Authors: | WANG Yue-jin XU Yan ZHANG Jian-xia ZHOU Peng WAN Yi-zhen |
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Institution: | 1. College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, P. R. China 2. National Key Laboratory of Biotechnology for Tropical Crops, CATAS, Haikou 571101 , P. R. China |
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Abstract: | With F1 individuals of the cross combination 88-110 of 83-4-96 ( V. quinquangularis Rehd. )× Muscat Rose ( V. vinifera L. ), the RAPD marker OPC15-1300 linked to ripe rot ( Gloeosporium fruetigenum Berk. ) resistance genes in Chinese wild Vitis was gained using bulked segregation analysis(BSA). And it was found that OPC15-1300 could be hereditary from the resistant parent (83-4-96) after the marker was tested in 50 F1 plants of the cross combination 88-110, 32 accessions of 8 Chinese wild Vitis species and 14cultivars of V. vinifera L. Also, it has provided a solid basis for molecular marker-assisted selection (MAS)and for possibly cloning disease resistance genes in the future. |
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Keywords: | Chinese wild Vitis Ripe rot resistance RAPD marker |
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